A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa
文献类型：期刊论文
| 作者 | Lu, Shiyou1,5; Li, Jianzhong2; Zhao, Huayan3; Lu, Wenying4,5; Feng, Tao5; Zhang, Lingling5; Wu, Pan4,5 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 19期号:12页码:15 |
| ISSN号 | 1422-0067 |
| 关键词 | Idesia polycarpa var glycine FAD2 linoleic acid oleic acid |
| DOI | 10.3390/ijms19123932 |
| 英文摘要 | High amounts of polyunsaturated fatty acids (PUFAs) in vegetable oil are not desirable for biodiesel or food oil due to their lower oxidative stability. The oil from Idesia polycarpa fruit contains 65-80% (mol%) linoleic acid (C18:2). Therefore, development of Idesia polycarpa cultivars with low PUFAs is highly desirable for Idesia polycarpa oil quality. Fatty acid desaturase 2 (FAD2) is the key enzyme converting oleic acid (C18:1) to C18:2. We isolated four FAD2 homologs from the fruit of Idesia polycarpa. Yeast transformed with IpFAD2-1, IpFAD2-2 and IpFAD2-3 can generate appreciable amounts of hexadecadienoic acid (C16:2) and C18:2, which are not present in wild-type yeast cells, revealing that the proteins encoded by these genes have (12) desaturase activity. Only trace amounts of C18:2 and little C16:2 were detected in yeast cells transformed with IpFAD2-4, suggesting IpFAD2-4 displays low activity. We also analyzed the activity of several FAD2 natural variants of Idesia polycarpa in yeast and found that a highly conserved Gly376 substitution caused the markedly reduced products catalyzed by IpFAD2-3. This glycine is also essential for the activity of IpFAD2-1 and IpFAD2-2, but its replacement in other plant FAD2 proteins displays different effects on the desaturase activity, suggesting its distinct roles across plant FAD2s proteins. |
| 资助项目 | Biological Resources Service Network from Chinese Academy of Sciences[kfj-brsn-2018-6-007] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000455323500234 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6487]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Lu, Shiyou |
| 作者单位 | 1.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 2.Tianjin Garrison Hangu Farm, Tianjin 300480, Peoples R China 3.Wuhan Inst Bioengn, Appl Biotechnol Ctr, Wuhan 430415, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Shiyou,Li, Jianzhong,Zhao, Huayan,et al. A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(12):15. |
| APA | Lu, Shiyou.,Li, Jianzhong.,Zhao, Huayan.,Lu, Wenying.,Feng, Tao.,...&Wu, Pan.(2018).A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(12),15. |
| MLA | Lu, Shiyou,et al."A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.12(2018):15. |
入库方式： OAI收割
来源：武汉植物园
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