Identification of Three (Iso)flavonoid Glucosyltransferases From Pueraria lobata
文献类型：期刊论文
| 作者 | Zhang, Yansheng2,3; Zhou, Zilin1,3; Li, Changfu2,3; Wang, Xin3 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2019-01-25 |
| 卷号 | 10页码:10 |
| ISSN号 | 1664-462X |
| 关键词 | glucosyltransferase Pueraria lobata flavonoids isoflavonoids daidzin |
| DOI | 10.3389/fpls.2019.00028 |
| 英文摘要 | (Iso)flavonoids are one of the largest groups of natural phenolic products conferring great value to the health of plants and humans. Pueraria lobata, a legume, has long been used in Chinese traditional medicine. (Iso)flavonoids mainly present as glycosyl-conjugates and accumulate in P. lobata roots. However, the molecular mechanism underlying the glycosylation processes in (iso)flavonoid biosynthesis are not fully understood. In the current study, three novel UDP-glycosyltransferases (PlUGT4, PlUGT15, and PlUGT57) were identified in P. lobata from RNA-seq data. Biochemical assays of these three recombinant PlUGTs showed all of them were able to glycosylate isoflavones (genistein and daidzein) at the 7-hydroxyl position in vitro. In comparison with the strict substrate specificity for PlUGT15 and PlUGT57, PlUGT4 displayed utilization of a broad range of sugar acceptors. Particularly, PlUGT15 exhibited a much higher catalytic efficiency toward isoflavones (genistein and daidzein) than any other identified 7-O-UGT from P. lobata. Moreover, the transcriptional expression patterns of these PlUGTs correlated with the accumulation of isoflavone glucosides in MeJA-treated P. lobata, suggesting their possible in vivo roles in the glycosylation process. |
| 资助项目 | National Natural Science Foundation of China[31170284] ; National Natural Science Foundation of China[31870275] ; One-Hundred Talents Program of Chinese Academy of Sciences[Y129441R01] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000456716400001 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6497]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Shanghai Univ, Sch Life Sci, Res Ctr Nat Prod, Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yansheng,Zhou, Zilin,Li, Changfu,et al. Identification of Three (Iso)flavonoid Glucosyltransferases From Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2019,10:10. |
| APA | Zhang, Yansheng,Zhou, Zilin,Li, Changfu,&Wang, Xin.(2019).Identification of Three (Iso)flavonoid Glucosyltransferases From Pueraria lobata.FRONTIERS IN PLANT SCIENCE,10,10. |
| MLA | Zhang, Yansheng,et al."Identification of Three (Iso)flavonoid Glucosyltransferases From Pueraria lobata".FRONTIERS IN PLANT SCIENCE 10(2019):10. |
入库方式： OAI收割
来源：武汉植物园
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