Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine
文献类型：期刊论文
| 作者 | Chen, Ze1,2; Chen, Quanjiao4; Lan, Jiaming3; Chen, Jie7; He, Bin7; Shao, Zhiyong7; Chen, Jianjun4; Wang, Huadong5,6; Yao, Yanfeng7,8 |
| 刊名 | EMERGING MICROBES & INFECTIONS
 |
| 出版日期 | 2019-01-16 |
| 卷号 | 8期号:1页码:45-54 |
| 关键词 | Influenza M2e tPA optimized M2e DNA vaccine Universal influenza vaccine |
| ISSN号 | 2222-1751 |
| DOI | 10.1080/22221751.2018.1558962 |
| 英文摘要 | Current influenza vaccines provide hemagglutinin strain-specific protection, but rarely provide cross-protection against divergent strains. It is, therefore, particularly important to develop a universal vaccine against conserved proteins or conserved regions of the virus. In this study, we used N-terminal extracellular region of the influenza virus M2 protein (M2e) as the target antigen and constructed two optimized M2e DNA vaccines (p-tPA-p3M2e and p-p3M2e) with increased antigenic epitope density and enhanced antigen secretion. Both vaccines induced high M2e-specific humoral and cellular immune responses in the vaccinated mice. These two vaccines also conferred protection against a lethal infection of homo-subtypic H1N1 virus, with p-tPA-p3M2e being the most effective. In addition, p-tPA-p3M2e also showed cross-protection against different subtypes of the influenza virus (H9N2, H6N6, and H10N8) at varying rates (80%, 40%, and 20%, respectively). After passive immunization, M2e DNA vaccine-induced antibodies in the sera provided complete protection against homologous virus challenge. An analysis of the mechanism underlying this immunization-mediated protection indicates that M2e-specific IgG and T-cell immune responses may play critical roles in the prevention of infection and viral clearance. Taken together, our results indicate that this optimized M2e DNA vaccine is a promising candidate for the development of a universal, broad-spectrum influenza virus vaccine. |
| 资助项目 | Wuhan Youth Science and Technology Plan[2016070204010104] ; China Mega-Project for Infectious Disease[2017ZX10103005-005] ; Hubei Provincial Natural Science Foundation of China[2016CFC743] ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding[KLAEMB-2016-06] ; National Natural Science Foundation of China[31570026] ; One-Three-Five Research Program of Wuhan Institute of Virology |
| WOS研究方向 | Immunology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000455807500006 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6512]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Jianjun |
| 作者单位 | 1.Shanghai Inst Biol Prod, Shanghai, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha, Hunan, Peoples R China 3.Hebei Med Univ, Dept Pathogen Biol, Shijiazhuang, Hebei, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Hubei, Peoples R China 6.Chinese Acad Sci, Wuhan Inst Phys & Math, Ctr Brain Sci, Key Lab Magnet Resonance Biol Syst, Wuhan, Hubei, Peoples R China 7.Wuhan Acad Agr Sci & Technol, Inst Anim Husb & Vet Sci, Wuhan, Hubei, Peoples R China 8.Chinese Acad Sci, Wuhan Inst Virol, Natl Biosafety Lab, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ze,Chen, Quanjiao,Lan, Jiaming,et al. Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine[J]. EMERGING MICROBES & INFECTIONS,2019,8(1):45-54. |
| APA | Chen, Ze.,Chen, Quanjiao.,Lan, Jiaming.,Chen, Jie.,He, Bin.,...&Yao, Yanfeng.(2019).Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine.EMERGING MICROBES & INFECTIONS,8(1),45-54. |
| MLA | Chen, Ze,et al."Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine".EMERGING MICROBES & INFECTIONS 8.1(2019):45-54. |
入库方式： OAI收割
来源：武汉植物园
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