Genetic Fate Mapping Defines the Vascular Potential of Endocardial Cells in the Adult Heart
文献类型:期刊论文
作者 | Tang, Juan1,2; Zhang, Hui1,2,3; He, Lingjuan1,2; Huang, Xiuzhen1,2; Li, Yan1,2; Pu, Wenjuan1,2; Yu, Wei1,2; Zhang, Libo1,2; Zhou, Bin1,2,3,4; Cai, Dongqing4 |
刊名 | CIRCULATION RESEARCH |
出版日期 | 2018 |
卷号 | 122期号:7页码:984-993 |
ISSN号 | 0009-7330 |
关键词 | Endothelial-cells Coronary-arteries Cardiac Neovascularization Angiogenesis Contributes Progenitors Form Medicine Injury |
DOI | 10.1161/CIRCRESAHA.117.312354 |
文献子类 | Article |
英文摘要 | Rationale: Endocardium is the major source of coronary endothelial cells (ECs) in the fetal and neonatal hearts. It remains unclear whether endocardium in the adult stage is also the main origin of neovascularization after cardiac injury. Objective: To define the vascular potential of adult endocardium in homeostasis and after cardiac injuries by fate-mapping studies. Methods and Results: We generate an inducible adult endocardial Cre line (Npr3 [natriuretic peptide receptor C]-CreER) and show that Npr3-CreER efficiently and specifically labels endocardial cells but not coronary blood vessels in the adult heart. The adult endocardial cells do not contribute to any vascular ECs during cardiac homeostasis. To examine the formation of blood vessels from endocardium after injury, we generate 4 cardiac injury models with Npr3-CreER mice: myocardial infarction, myocardial ischemia-reperfusion, cryoinjury, and transverse aortic constriction. Lineage tracing experiments show that adult endocardium minimally contributes to coronary ECs after myocardial infarction. In the myocardial ischemia-reperfusion, cryoinjury, or transverse aortic constriction models, adult endocardial cells do not give rise to any vascular ECs, and they remain on the inner surface of myocardium that connects with lumen circulation. In the myocardial infarction model, very few endocardial cells are trapped in the infarct zone of myocardium shortly after ligation of coronary artery, indicating the involvement of endocardial entrapment during blood vessels formation. When these adult endocardial cells are relocated and trapped in the infarcted myocardium by transplantation or myocardial constriction model, very few endocardial cells survive and gain vascular EC properties, and their contribution to neovascularization in the injured myocardium remains minimal. Conclusions: Unlike its fetal or neonatal counterpart, adult endocardium naturally generates minimal, if any, coronary arteries or vascular ECs during cardiac homeostasis or after injuries. |
电子版国际标准刊号 | 1524-4571 |
WOS研究方向 | Cardiac & Cardiovascular Systems ; Hematology ; Peripheral Vascular Disease |
语种 | 英语 |
WOS记录号 | WOS:000428709500017 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3407] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Zhou, Bin |
作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Beijing, Peoples R China; 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Beijing, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 4.Jinan Univ, Inst Aging & Regenerat Med, Key Lab Regenerat Med, Minist Educ, Guangzhou, Guangdong, Peoples R China; 5.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China |
推荐引用方式 GB/T 7714 | Tang, Juan,Zhang, Hui,He, Lingjuan,et al. Genetic Fate Mapping Defines the Vascular Potential of Endocardial Cells in the Adult Heart[J]. CIRCULATION RESEARCH,2018,122(7):984-993. |
APA | Tang, Juan.,Zhang, Hui.,He, Lingjuan.,Huang, Xiuzhen.,Li, Yan.,...&Lui, Kathy O..(2018).Genetic Fate Mapping Defines the Vascular Potential of Endocardial Cells in the Adult Heart.CIRCULATION RESEARCH,122(7),984-993. |
MLA | Tang, Juan,et al."Genetic Fate Mapping Defines the Vascular Potential of Endocardial Cells in the Adult Heart".CIRCULATION RESEARCH 122.7(2018):984-993. |
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