中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Genetic targeting of Purkinje fibres by Sema3a-CreERT2

文献类型:期刊论文

作者Li, Yan1,2; Tian, Xueying1,2; Zhao, Huan1,2; He, Lingjuan1,2; Zhang, Shaohua1,2; Huang, Xiuzhen1,2; Zhang, Hui1,2; Zhou, Bin1,2,4,5; Miquerol, Lucile3
刊名SCIENTIFIC REPORTS
出版日期2018
卷号8期号:1页码:2382
ISSN号2045-2322
关键词Ventricular Conduction System Nervous-system Heart Angiogenesis Semaphorins
DOI10.1038/s41598-018-20829-9
文献子类Article
英文摘要

The maintenance of the heart rhythm and the conduction of excitatory signals require changing excitatory signals via electrical activity and coordination by communication between working and conductive cardiomyocytes. Understanding how the ventricular conduction system is established provides novel insights into the pathophysiological progress of cardiac arrhythmias. However, the major hurdle in this field is the lack of a specific genetic tool that targets the Purkinje fibres of the ventricular conduction system and no other types of cardiomyocytes or coronary vessels. Here, we generated a Sema3a-CreERT2 knock-in mouse line to test its specificity for genetically labelled Purkinje fibres. We found that Sema3a was expressed in the subendocardial layer of the trabecular myocardium in the embryonic heart and was restricted to the Purkinje fibres in the adult heart. A fate mapping study based on the Sema3a-CreERT2 line revealed that the Sema3a(+) cardiomyocytes were restricted to the fate of Purkinje fibres in the perinatal but not the embryonic stage. Collectively, our study provides a new genetic tool, i.e., Sema3a-CreERT2, for studying the molecular mechanisms that regulate the function of Purkinje fibres.

WOS研究方向Multidisciplinary Sciences
语种英语
WOS记录号WOS:000424087700054
版本出版稿
源URL[http://202.127.25.143/handle/331003/3411]  
专题生化所2018年发文
通讯作者Zhou, Bin
作者单位1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai 200031, Peoples R China;
2.Univ Chinese Acad Sci Shanghai, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China;
3.Aix Marseille Univ, CNRS, IBDM, UMR 7288, F-13288 Marseille, France;
4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou 510632, Guangdong, Peoples R China;
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式
GB/T 7714
Li, Yan,Tian, Xueying,Zhao, Huan,et al. Genetic targeting of Purkinje fibres by Sema3a-CreERT2[J]. SCIENTIFIC REPORTS,2018,8(1):2382.
APA Li, Yan.,Tian, Xueying.,Zhao, Huan.,He, Lingjuan.,Zhang, Shaohua.,...&Miquerol, Lucile.(2018).Genetic targeting of Purkinje fibres by Sema3a-CreERT2.SCIENTIFIC REPORTS,8(1),2382.
MLA Li, Yan,et al."Genetic targeting of Purkinje fibres by Sema3a-CreERT2".SCIENTIFIC REPORTS 8.1(2018):2382.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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