Genetic targeting of Purkinje fibres by Sema3a-CreERT2
文献类型:期刊论文
作者 | Li, Yan1,2; Tian, Xueying1,2; Zhao, Huan1,2; He, Lingjuan1,2; Zhang, Shaohua1,2; Huang, Xiuzhen1,2; Zhang, Hui1,2; Zhou, Bin1,2,4,5![]() |
刊名 | SCIENTIFIC REPORTS
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出版日期 | 2018 |
卷号 | 8期号:1页码:2382 |
关键词 | Ventricular Conduction System Nervous-system Heart Angiogenesis Semaphorins |
ISSN号 | 2045-2322 |
DOI | 10.1038/s41598-018-20829-9 |
文献子类 | Article |
英文摘要 | The maintenance of the heart rhythm and the conduction of excitatory signals require changing excitatory signals via electrical activity and coordination by communication between working and conductive cardiomyocytes. Understanding how the ventricular conduction system is established provides novel insights into the pathophysiological progress of cardiac arrhythmias. However, the major hurdle in this field is the lack of a specific genetic tool that targets the Purkinje fibres of the ventricular conduction system and no other types of cardiomyocytes or coronary vessels. Here, we generated a Sema3a-CreERT2 knock-in mouse line to test its specificity for genetically labelled Purkinje fibres. We found that Sema3a was expressed in the subendocardial layer of the trabecular myocardium in the embryonic heart and was restricted to the Purkinje fibres in the adult heart. A fate mapping study based on the Sema3a-CreERT2 line revealed that the Sema3a(+) cardiomyocytes were restricted to the fate of Purkinje fibres in the perinatal but not the embryonic stage. Collectively, our study provides a new genetic tool, i.e., Sema3a-CreERT2, for studying the molecular mechanisms that regulate the function of Purkinje fibres. |
WOS研究方向 | Multidisciplinary Sciences |
语种 | 英语 |
WOS记录号 | WOS:000424087700054 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3411] ![]() |
专题 | 生化所2018年发文 |
通讯作者 | Zhou, Bin |
作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai 200031, Peoples R China; 2.Univ Chinese Acad Sci Shanghai, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China; 3.Aix Marseille Univ, CNRS, IBDM, UMR 7288, F-13288 Marseille, France; 4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou 510632, Guangdong, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Yan,Tian, Xueying,Zhao, Huan,et al. Genetic targeting of Purkinje fibres by Sema3a-CreERT2[J]. SCIENTIFIC REPORTS,2018,8(1):2382. |
APA | Li, Yan.,Tian, Xueying.,Zhao, Huan.,He, Lingjuan.,Zhang, Shaohua.,...&Miquerol, Lucile.(2018).Genetic targeting of Purkinje fibres by Sema3a-CreERT2.SCIENTIFIC REPORTS,8(1),2382. |
MLA | Li, Yan,et al."Genetic targeting of Purkinje fibres by Sema3a-CreERT2".SCIENTIFIC REPORTS 8.1(2018):2382. |
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