In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity
文献类型:期刊论文
作者 | Zhang, Kun1,2; Peng, Luying1; Zhang, Ludi2; Liu, Wenming2; Ma, Xiaolong2; Cen, Jin2; Sun, Zhen2,3; Wang, Chenhua2; Feng, Sisi2; Zhang, Zhengtao2 |
刊名 | CELL STEM CELL |
出版日期 | 2018 |
卷号 | 23期号:6页码:806-+ |
ISSN号 | 1934-5909 |
关键词 | Long-term Culture Stem-cells Gene-expression Conversion Fibroblasts Progenitors Arrest Wnt |
DOI | 10.1016/j.stem.2018.10.018 |
文献子类 | Article |
英文摘要 | Transplantation of human hepatocytes (HHs) holds significant potential for treating liver diseases. However, the supply of transplantable HHs is severely constrained by limited donor availability and compromised capacity for in vitro expansion. In response to chronic injury, some HHs are reprogrammed into proliferative cells that express both hepatocyte and progenitor markers, suggesting exploitable strategies for expanding HHs in vitro. Here, we report defined medium conditions that allow 10,000-fold expansion of HHs. These proliferating HHs are bi-phenotypic, partially retaining hepatic features while gaining expression of progenitor-associated genes. Importantly, these cells engraft into injured mouse liver at a level comparable to primary HHs, and they undergo maturation following transplantation in vivo or differentiation in vitro. Thus, this study provides a protocol that enables large-scale expansion of transplantable HHs, which could be further developed for modeling and treating human liver disease. |
电子版国际标准刊号 | 1875-9777 |
WOS研究方向 | Cell & Tissue Engineering ; Cell Biology |
语种 | 英语 |
WOS记录号 | WOS:000452550400009 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3424] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Peng, Luying; Liu, Wenming; Hui, Lijian; Zhu, Zhenfeng |
作者单位 | 1.Tongji Univ, Shanghai East Hosp, Key Lab Arrhythmias, Minist Educ,Sch Med, Shanghai 200120, Peoples R China; 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Cell Biol,Shanghai Inst Biochem & C, Shanghai 200031, Peoples R China; 3.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Fudan Univ, Shanghai Med Coll, Dept Oncol, Dept Integrat Oncol,Fudan Univ Shanghai Canc Ctr, Shanghai 200032, Peoples R China; 5.Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China; 6.Stem Cell & Regenerat Med Innovat Acad, Beijing 100101, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Kun,Peng, Luying,Zhang, Ludi,et al. In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity[J]. CELL STEM CELL,2018,23(6):806-+. |
APA | Zhang, Kun.,Peng, Luying.,Zhang, Ludi.,Liu, Wenming.,Ma, Xiaolong.,...&Li, Peng.(2018).In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity.CELL STEM CELL,23(6),806-+. |
MLA | Zhang, Kun,et al."In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity".CELL STEM CELL 23.6(2018):806-+. |
入库方式: OAI收割
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