Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation
文献类型:期刊论文
作者 | Friedman, Clayton E.1,2; Quan Nguyen1; Lukowski, Samuel W.1; Helfer, Abbigail1,2; Chiu, Han Sheng1,2; Baillie, Greg J.1; Senabouth, Anne1; Christ, Angelika N.1; Bruxner, Timothy J.1; Powell, Joseph E.1,15 |
刊名 | CELL STEM CELL |
出版日期 | 2018 |
卷号 | 23期号:4页码:586-+ |
ISSN号 | 1934-5909 |
关键词 | Pluripotent Stem-cells Gene-expression Homeodomain Protein Rna-seq Hypertrophy Modulation Mouse |
DOI | 10.1016/j.stem.2018.09.009 |
文献子类 | Article |
英文摘要 | Cardiac differentiation of human pluripotent stem cells (hPSCs) requires orchestration of dynamic gene regulatory networks during stepwise fate transitions but often generates immature cell types that do not fully recapitulate properties of their adult counterparts, suggesting incomplete activation of key transcriptional networks. We performed extensive single-cell transcriptomic analyses to map fate choices and gene expression programs during cardiac differentiation of hPSCs and identified strategies to improve in vitro cardiomyocyte differentiation. Utilizing genetic gain- and loss-of-function approaches, we found that hypertrophic signaling is not effectively activated during monolayer-based cardiac differentiation, thereby preventing expression of HOPX and its activation of downstream genes that govern late stages of cardiomyocyte maturation. This study therefore provides a key transcriptional roadmap of in vitro cardiac differentiation at single-cell resolution, revealing fundamental mechanisms underlying heart development and differentiation of hPSC-derived cardiomyocytes. |
电子版国际标准刊号 | 1875-9777 |
WOS研究方向 | Cell & Tissue Engineering ; Cell Biology |
语种 | 英语 |
WOS记录号 | WOS:000446342800017 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3468] |
专题 | 生化所2018年发文 |
通讯作者 | Powell, Joseph E.; Palpant, Nathan J. |
作者单位 | 1.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia; 2.Univ Queensland, Ctr Cardiac & Vasc Biol, Brisbane, Qld 4072, Australia; 3.Univ Queensland, Sch Biomed Sci, Brisbane, Qld 4072, Australia; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Max Planck Partner Inst Computat Biol, Key Lab Computat Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China; 5.Univ Sydney, Childrens Med Res Inst, Embryol Unit, Westmead, NSW 2145, Australia; 6.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China; 7.Univ Chinese Acad Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China; 8.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China; 9.Univ Washington, Inst Stem Cell & Regenerat Med, Dept Pathol, Seattle, WA 98195 USA; 10.Univ Washington, Inst Stem Cell & Regenerat Med, Dept Biochem, Seattle, WA 98195 USA; |
推荐引用方式 GB/T 7714 | Friedman, Clayton E.,Quan Nguyen,Lukowski, Samuel W.,et al. Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation[J]. CELL STEM CELL,2018,23(4):586-+. |
APA | Friedman, Clayton E..,Quan Nguyen.,Lukowski, Samuel W..,Helfer, Abbigail.,Chiu, Han Sheng.,...&Hudson, James.(2018).Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation.CELL STEM CELL,23(4),586-+. |
MLA | Friedman, Clayton E.,et al."Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation".CELL STEM CELL 23.4(2018):586-+. |
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