Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells
文献类型:期刊论文
作者 | Liu, Chang1; Wang, Ran1; Yang, Xianfa1,5; Chen, Jun1; Cui, Guizhong1; Guo, Wenke1,5; Chen, Yingying1,5; Peng, Guangdun1; Jing, Naihe1,5; He, Zhisong2 |
刊名 | STEM CELL REPORTS |
出版日期 | 2018 |
卷号 | 11期号:1页码:43-57 |
ISSN号 | 2213-6711 |
关键词 | Mouse Embryo Visceral Endoderm Axis Formation Primitive Streak Rna-seq Gastrulation Pluripotency Requirement Embryogenesis Morphogenesis |
DOI | 10.1016/j.stemcr.2018.05.019 |
文献子类 | Article |
英文摘要 | The molecular mechanism underpinning the specification of the ectoderm, a transient germ-layer tissue, during mouse gastrulation was examined here in a stem cell-based model. We captured a self-renewing cell population with enhanced ectoderm potency from mouse epiblast stem cells (EpiSCs) by suppressing Nodal signaling activity. The transcriptome of the Nodal-inhibited EpiSCs resembles that of the anterior epiblast of embryonic day (E)7.0 and E7.5 mouse embryo, which is accompanied by chromatin modifications that reflect the priming of ectoderm lineage-related genes for expression. Nodal-inhibited EpiSCs show enhanced ectoderm differentiation in vitro and contribute to the neuroectoderm and the surface ectoderm in postimplantation chimeras but lose the propensity for mesendoderm differentiation in vitro and in chimeras. Our findings show that specification of the ectoderm progenitors is enhanced by the repression of Nodal signaling activity, and the ectoderm-like stem cells provide an experimental model to investigate the molecular characters of the epiblast-derived ectoderm. |
WOS研究方向 | Cell & Tissue Engineering ; Cell Biology |
语种 | 英语 |
WOS记录号 | WOS:000438067600006 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3484] |
专题 | 生化所2018年发文 |
通讯作者 | Jing, Naihe |
作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, 320 Yue Yang Rd, Shanghai 200031, Peoples R China; 2.CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai 200031, Peoples R China; 3.Childrens Med Res Inst, Embryol Unit, Westmead, NSW 2145, Australia; 4.Childrens Med Res Inst, Bioinformat Grp, Westmead, NSW 2145, Australia; 5.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China; 6.Univ Sydney, Sydney Med Sch, Sch Med Sci, Westmead, NSW 2145, Australia |
推荐引用方式 GB/T 7714 | Liu, Chang,Wang, Ran,Yang, Xianfa,et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells[J]. STEM CELL REPORTS,2018,11(1):43-57. |
APA | Liu, Chang.,Wang, Ran.,Yang, Xianfa.,Chen, Jun.,Cui, Guizhong.,...&Tam, Patrick P. L..(2018).Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells.STEM CELL REPORTS,11(1),43-57. |
MLA | Liu, Chang,et al."Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells".STEM CELL REPORTS 11.1(2018):43-57. |
入库方式: OAI收割
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