The TEA domain family transcription factor TEAD4 represses murine adipogenesis by recruiting the cofactors VGLL4 and CtBP2 into a transcriptional complex
文献类型:期刊论文
作者 | Zhang, Wenxiang1; Xu, Jinjin1; Li, Jinhui1; Guo, Tong1; Feng, Xue1; Ma, Xueyan1; He, Lingli1; Wu, Wenqing1; Yin, Mengxin1; Ge, Ling1 |
刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
出版日期 | 2018 |
卷号 | 293期号:44页码:17119-17134 |
ISSN号 | 0021-9258 |
关键词 | Activated Receptor-gamma Kruppel-like Factor Organ Size Control Large Gene Lists Adipocyte Differentiation Signaling Pathway Stem-cells Functional-analysis Binding Domain Gastric-cancer |
DOI | 10.1074/jbc.RA118.003608 |
文献子类 | Article |
英文摘要 | The Hippo signaling pathway is known to play an important role in multiple physiological processes, including adipogenesis. However, whether the downstream components of the Hippo pathway are involved in adipogenesis remains unknown. Here we demonstrate that the TEA domain family (TEAD) transcription factors are essential for adipogenesis in murine 3T3-L1 preadipocytes. Knockdown of TEAD1-4 stimulated adipogenesis and increased the expression of adipocyte markers in these cells. Interestingly, we found that the TEAD4 knockdown-mediated adipogenesis proceeded in a Yes-associated protein (YAP)/TAZ (Wwtr1)-independent manner and that adipogenesis suppression in WT cells involved formation of a ternary complex comprising TEAD4 and the transcriptional cofactors C-terminal binding protein 2 (CtBP2) and vestigial-like family member 4 (VGLL4). VGLL4 acted as an adaptor protein that enhanced the interaction between TEAD4 and CtBP2, and this TEAD4-VGLL4-CtBP2 ternary complex dynamically existed at the early stage of adipogenesis. Finally, we verified that TEAD4 directly targets the promoters of major adipogenesis transcription factors such as peroxisome proliferator-activated receptor (PPAR) and adiponectin, C1Q, and collagen domain-containing (Adipoq) during adipogenesis. These findings reveal critical insights into the role of the TEAD4-VGLL4-CtBP2 transcriptional repressor complex in suppression of adipogenesis in murine preadipocytes. |
电子版国际标准刊号 | 1083-351X |
WOS研究方向 | Biochemistry & Molecular Biology |
语种 | 英语 |
WOS记录号 | WOS:000449466500014 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3503] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Fei, Zhaoliang |
作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,Innovat Ctr Cel, State Key Lab Cell Biol,Chinese Acad Sci Ctr Exce, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Wenxiang,Xu, Jinjin,Li, Jinhui,et al. The TEA domain family transcription factor TEAD4 represses murine adipogenesis by recruiting the cofactors VGLL4 and CtBP2 into a transcriptional complex[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(44):17119-17134. |
APA | Zhang, Wenxiang.,Xu, Jinjin.,Li, Jinhui.,Guo, Tong.,Feng, Xue.,...&Ho, Margaret S..(2018).The TEA domain family transcription factor TEAD4 represses murine adipogenesis by recruiting the cofactors VGLL4 and CtBP2 into a transcriptional complex.JOURNAL OF BIOLOGICAL CHEMISTRY,293(44),17119-17134. |
MLA | Zhang, Wenxiang,et al."The TEA domain family transcription factor TEAD4 represses murine adipogenesis by recruiting the cofactors VGLL4 and CtBP2 into a transcriptional complex".JOURNAL OF BIOLOGICAL CHEMISTRY 293.44(2018):17119-17134. |
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