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Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii

文献类型：期刊论文

作者Li, Xuegong1,3; Fu, Ling3; Li, Zhen3; Xu, Jun2,3,4; Xiao, Xiang2,3,4; Ma, Xiaopan3
刊名EXTREMOPHILES
出版日期2015
卷号19期号:1页码:59-67
关键词Piezophile Genetic Tool Pyrococcus Yayanosii Hyperthermophilic Archaeon
DOI10.1007/s00792-014-0705-2
文献子类Article
英文摘要The hyperthermophile Pyrococcus yayanosii CH1 is the only high-pressure-requiring microorganism obtained thus far within the archaea domain or among all non-psychrophiles in any domain. In this study, we developed a genetic manipulation system for P. yayanosii after first isolating a facultatively piezophilic derivative strain, designated P. yayanosii A1. The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene was overexpressed in strain P. yayanosii A1 and was demonstrated to confer host cell resistance against simvastatin. Furthermore, using simvastatin as a selection marker, the endogenous pyrF of P. yayanosii A1 was disrupted through homologous recombination, thus generating the additional host strain P. yayanosii A2 (Delta pyrF). A markerless gene disruption vector was constructed by incorporating a pyrF-sim (R) cassette that enables the combined use of simvastatin resistance for positive selection and 5-FOA for counter selection. The utility of this versatile disruption system was demonstrated by deleting the carbon-nitrogen hydrolase of P. yayanosii strain A1. These results demonstrate that a variety of genetic tools are now in place to study unknown gene function and the molecular mechanisms of piezophilic adaptation in P. yayanosii.
学科主题海洋生物学
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WOS关键词Piezophile ; Genetic tool ; Pyrococcus yayanosii ; Hyperthermophilic archaeon
语种英语
WOS记录号WOS:000346657500006
版本出版稿
源URL[http://ir.idsse.ac.cn/handle/183446/1204]  
专题深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
作者单位1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Dept Deep Sea Sci, Sanya 572000, Peoples R China
2.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
3.Shanghai Jiao Tong Univ, Inst Oceanol, Shanghai 200240, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
推荐引用方式
GB/T 7714		 Li, Xuegong,Fu, Ling,Li, Zhen,et al. Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii[J]. EXTREMOPHILES,2015,19(1):59-67.
APA Li, Xuegong,Fu, Ling,Li, Zhen,Xu, Jun,Xiao, Xiang,&Ma, Xiaopan.(2015).Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii.EXTREMOPHILES,19(1),59-67.
MLA Li, Xuegong,et al."Genetic tools for the piezophilic hyperthermophilic archaeon Pyrococcus yayanosii".EXTREMOPHILES 19.1(2015):59-67.

入库方式： OAI收割

来源：深海科学与工程研究所

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