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Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp

文献类型：期刊论文

作者Wang, Yibin1,2; He, Yingying1; Xing, Lei7; Zhang, Xiufang3; Wang, Shuai1,6; Qi, Xiaoqing1,5; Zheng, Zhou1,2; Lu, Jian4; Miao, Jinlai1,2; Miao, Jinlai(State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China)
刊名3 BIOTECH
出版日期2017-09-22
卷号7
关键词Trehalose Antarctic Pseudozyma Sp. Genome Trehalose Phosphate Synthase
DOI10.1007/s13205-017-0983-3
产权排序第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位 ; 第5完成单位 ; 第6完成单位 ; 第7完成单位
文献子类Article
英文摘要Trehalose is a non-reducing disaccharide sugar that widely exists in a variety of organisms, such as bacteria and eukaryotes except the vertebrates. It plays an important role in a number of critical metabolic functions especially in response to stressful environmental conditions. However, the biosynthetic pathways of trehalose in cold-adapted yeast and its responses to temperature and salinity changes remain little understood. In this study, the genome of Antarctic-isolated Pseudozyma sp. NJ7 was generated from which we identified the gene coding for trehalose phosphate synthase (TPS1) and trehalose phosphate phosphatase (TPS2), the two enzymes most critical for trehalose production. The whole draft genome length of Pseudozyma sp. NJ7 was 18,021,233 bp, and encoded at least 34 rRNA operons and 72 tRNAs. The open reading frame of tps1 contained 1827 nucleotide encoding 608 amino acids with a molecular weight of 67.64 kDa, and an isoelectric point of 5.54, while tps2 contained 3948 nucleotide encoding 1315 amino acids with a molecular weight of 144.47 kDa and an isoelectric point of 6.36. The TPS1 and TPS2 protein sequences were highly homologous to Moesziomyces antarcticus T-34, but TPS2 had obvious specificity and differently with others which suggest species specificity and different evolutionary history. Expression level of tps1 gene was strongly influenced by temperature and high salinity. In addition, addition of 0.5% trehalose preserved yeast cells in the short term but was not effective for cryopreservation for more than 5 days, but still suggesting that exogenous trehalose could indeed significantly improve the survival of yeast cells under freezing conditions. Our results provided new insights on the molecular basis of cold adaptations of Antarctic Pseudozyma sp., and also generated new information on the roles trehalose play in yeast tolerance to extreme conditions in the extreme Antarctic environments.
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WOS关键词TREHALOSE-6-PHOSPHATE SYNTHASE GENE ; SACCHAROMYCES-CEREVISIAE ; FREEZE TOLERANCE ; BAKERS-YEAST ; TEMPERATURES ; BIOSYNTHESIS ; 6-PHOSPHATE ; METABOLISM ; SEQUENCE ; PATHWAY
语种英语
WOS记录号WOS:000411736100001
源URL[http://ir.idsse.ac.cn/handle/183446/4771]  
专题深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
通讯作者Miao, Jinlai; Miao, Jinlai(State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China)
作者单位1.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266235, Peoples R China
3.Qingdao Hiser Med Ctr, Clin Lab, Qingdao 266033, Peoples R China
4.Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
6.Marine & Fisheries Monitoring Ctr Sanya, Sanya 572000, Peoples R China
7.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266061, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yibin,He, Yingying,Xing, Lei,et al. Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp[J]. 3 BIOTECH,2017,7.
APA Wang, Yibin.,He, Yingying.,Xing, Lei.,Zhang, Xiufang.,Wang, Shuai.,...&Yin, Hua.(2017).Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp.3 BIOTECH,7.
MLA Wang, Yibin,et al."Cloning and expression analysis of tps, and cryopreservation research of trehalose from Antarctic strain Pseudozyma sp".3 BIOTECH 7(2017).

入库方式： OAI收割

来源：深海科学与工程研究所

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