中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite

文献类型:期刊论文

作者Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China); He, Li-Sheng1,2; Qian, Pei-Yuan1; Zhang, Gen1; Wang, Yong1,2; Yan, Guo-Yong2
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2018
卷号495期号:1页码:969-975
关键词Barnacle Cement Protein Cp100k Evolution Settlement
DOI10.1016/j.bbrc.2017.11.101
产权排序第1完成单位 ; 第2完成单位
文献子类Article
英文摘要Barnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized from Amphibalanus amphitrite in this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles including Megabalanus rosa (Mr-cp100k), Tetraclita japonica formosana (Tj-cp100k) and Pollicipes pollicipes (Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid beta secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that in Megabalanus rosa, implying diverse possible roles of cp100k in cementing. (C) 2017 Published by Elsevier Inc.
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WOS关键词BALANUS-AMPHITRITE ; UNDERWATER ADHESIVE ; MEGABALANUS-ROSA ; SERINE-PROTEASE ; CYPRIS LARVA ; IN-SITU ; PROTEINS ; BALANOIDES ; EXOCYTOSIS ; MICROSCOPY
语种英语
WOS记录号WOS:000423897600146
源URL[http://ir.idsse.ac.cn/handle/183446/5926]  
专题深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China); Qian, Pei-Yuan
作者单位1.Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China
2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China
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Qian, Pei-Yuan,He, Li-Sheng,Qian, Pei-Yuan,et al. Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(1):969-975.
APA Qian, Pei-Yuan,He, Li-Sheng,Qian, Pei-Yuan,Zhang, Gen,Wang, Yong,&Yan, Guo-Yong.(2018).Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(1),969-975.
MLA Qian, Pei-Yuan,et al."Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.1(2018):969-975.

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来源:深海科学与工程研究所

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