Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite
文献类型:期刊论文
作者 | Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China); He, Li-Sheng1,2![]() ![]() ![]() |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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出版日期 | 2018 |
卷号 | 495期号:1页码:969-975 |
关键词 | Barnacle Cement Protein Cp100k Evolution Settlement |
DOI | 10.1016/j.bbrc.2017.11.101 |
产权排序 | 第1完成单位 ; 第2完成单位 |
文献子类 | Article |
英文摘要 | Barnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized from Amphibalanus amphitrite in this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles including Megabalanus rosa (Mr-cp100k), Tetraclita japonica formosana (Tj-cp100k) and Pollicipes pollicipes (Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid beta secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that in Megabalanus rosa, implying diverse possible roles of cp100k in cementing. (C) 2017 Published by Elsevier Inc. |
URL标识 | 查看原文 |
WOS关键词 | BALANUS-AMPHITRITE ; UNDERWATER ADHESIVE ; MEGABALANUS-ROSA ; SERINE-PROTEASE ; CYPRIS LARVA ; IN-SITU ; PROTEINS ; BALANOIDES ; EXOCYTOSIS ; MICROSCOPY |
语种 | 英语 |
WOS记录号 | WOS:000423897600146 |
源URL | [http://ir.idsse.ac.cn/handle/183446/5926] ![]() |
专题 | 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 |
通讯作者 | Qian, Pei-Yuan(Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China); Qian, Pei-Yuan |
作者单位 | 1.Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China |
推荐引用方式 GB/T 7714 | Qian, Pei-Yuan,He, Li-Sheng,Qian, Pei-Yuan,et al. Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(1):969-975. |
APA | Qian, Pei-Yuan,He, Li-Sheng,Qian, Pei-Yuan,Zhang, Gen,Wang, Yong,&Yan, Guo-Yong.(2018).Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(1),969-975. |
MLA | Qian, Pei-Yuan,et al."Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.1(2018):969-975. |
入库方式: OAI收割
来源:深海科学与工程研究所
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