中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina

文献类型:期刊论文

作者Wong, Yue Him2,3; Yu, Li2; Zhang, Gen2; He, Li-Sheng1,2; Qian, Pei-Yuan1,2
刊名PLOS ONE
出版日期2016-08-18
卷号11期号:8
DOI10.1371/journal.pone.0160271
文献子类Article
英文摘要The bryozoan Bugula neritina has a biphasic life cycle that consists of a planktonic larval stage and a sessile juvenile/adult stage. The transition between these two stages is crucial for the development and recruitment of B. neritina. Metamorphosis in B. neritina is mediated by both the nervous system and the release of developmental signals. However, no research has been conducted to investigate the expression of neuropeptides (NP)/peptide hormones in B. neritina larvae. Here, we report a comprehensive study of the NP/peptide hormones in the marine bryozoan B. neritina based on in silico identification methods. We recovered 22 transcripts encompassing 11 NP/peptide hormone precursor transcript sequences. The transcript sequences of the 11 isolated NP precursors were validated by cDNA cloning using gene-specific primers. We also examined the expression of three peptide hormone precursor transcripts (BnFDSIG, BnILP1, BnGPB) in the coronate larvae of B. neritina, demonstrating their distinct expression patterns in the larvae. Overall, our findings serve as an important foundation for subsequent investigations of the peptidergic control of bryozoan larval behavior and settlement.
WOS关键词COCKROACH LEUCOPHAEA-MADERAE ; LARVAL NERVOUS-SYSTEM ; MASS-SPECTROMETRY ; SEQUENCING TECHNOLOGIES ; PEDAL PEPTIDE ; BRAIN ; IDENTIFICATION ; CELLULARIOIDEA ; METAMORPHOSIS ; EVOLUTIONARY
语种英语
WOS记录号WOS:000381577000016
源URL[http://ir.idsse.ac.cn/handle/183446/1608]  
专题深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
作者单位1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, San Ya, Hai Nan, Peoples R China
2.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
3.Akita Prefectural Univ, Dept Biotechnol, Akita, Japan
推荐引用方式
GB/T 7714
Wong, Yue Him,Yu, Li,Zhang, Gen,et al. In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina[J]. PLOS ONE,2016,11(8).
APA Wong, Yue Him,Yu, Li,Zhang, Gen,He, Li-Sheng,&Qian, Pei-Yuan.(2016).In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina.PLOS ONE,11(8).
MLA Wong, Yue Him,et al."In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina".PLOS ONE 11.8(2016).

入库方式: OAI收割

来源:深海科学与工程研究所

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