In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina
文献类型：期刊论文
| 作者 | Wong, Yue Him2,3; Yu, Li2; Zhang, Gen2; He, Li-Sheng1,2 ; Qian, Pei-Yuan1,2
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| 刊名 | PLOS ONE
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| 出版日期 | 2016-08-18 |
| 卷号 | 11期号:8 |
| DOI | 10.1371/journal.pone.0160271 |
| 文献子类 | Article |
| 英文摘要 | The bryozoan Bugula neritina has a biphasic life cycle that consists of a planktonic larval stage and a sessile juvenile/adult stage. The transition between these two stages is crucial for the development and recruitment of B. neritina. Metamorphosis in B. neritina is mediated by both the nervous system and the release of developmental signals. However, no research has been conducted to investigate the expression of neuropeptides (NP)/peptide hormones in B. neritina larvae. Here, we report a comprehensive study of the NP/peptide hormones in the marine bryozoan B. neritina based on in silico identification methods. We recovered 22 transcripts encompassing 11 NP/peptide hormone precursor transcript sequences. The transcript sequences of the 11 isolated NP precursors were validated by cDNA cloning using gene-specific primers. We also examined the expression of three peptide hormone precursor transcripts (BnFDSIG, BnILP1, BnGPB) in the coronate larvae of B. neritina, demonstrating their distinct expression patterns in the larvae. Overall, our findings serve as an important foundation for subsequent investigations of the peptidergic control of bryozoan larval behavior and settlement. |
| WOS关键词 | COCKROACH LEUCOPHAEA-MADERAE ; LARVAL NERVOUS-SYSTEM ; MASS-SPECTROMETRY ; SEQUENCING TECHNOLOGIES ; PEDAL PEPTIDE ; BRAIN ; IDENTIFICATION ; CELLULARIOIDEA ; METAMORPHOSIS ; EVOLUTIONARY |
| 语种 | 英语 |
| WOS记录号 | WOS:000381577000016 |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/1608]  |
| 专题 | 深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组 |
| 作者单位 | 1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, San Ya, Hai Nan, Peoples R China 2.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China 3.Akita Prefectural Univ, Dept Biotechnol, Akita, Japan |
| 推荐引用方式 GB/T 7714 | Wong, Yue Him,Yu, Li,Zhang, Gen,et al. In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina[J]. PLOS ONE,2016,11(8). |
| APA | Wong, Yue Him,Yu, Li,Zhang, Gen,He, Li-Sheng,&Qian, Pei-Yuan.(2016).In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina.PLOS ONE,11(8). |
| MLA | Wong, Yue Him,et al."In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina".PLOS ONE 11.8(2016). |
入库方式： OAI收割
来源：深海科学与工程研究所
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