Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
文献类型：期刊论文
| 作者 | Sun, Jin3; Lan, Yi3; Zhang, Gen1; Yan, Guoyong2,4 ; Huang, Jiaomei2; He, Lisheng(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China); He, Lisheng2; Qian, Pei-Yuan3; Wang, Yong2; Zeng, Cong2
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2017-11-01 |
| 卷号 | 18期号:11 |
| 关键词 | Megabalanus Volcano Transcriptome Larval Settlement Neuropeptide Opsin Gpcrs |
| DOI | 10.3390/ijms18112253 |
| 产权排序 | 第1完成单位 ; 第2完成单位 ; 第3完成单位 ; 第4完成单位 |
| 文献子类 | Article |
| 英文摘要 | Megabalanus barnacle is one of the model organisms for marine biofouling research. However, further elucidation of molecular mechanisms underlying larval settlement has been hindered due to the lack of genomic information thus far. In the present study, cDNA libraries were constructed for cyprids, the key stage for larval settlement, and adults of Megabalanus volcano. After high-throughput sequencing and de novo assembly, 42,620 unigenes were obtained with a N50 value of 1532 bp. These unigenes were annotated by blasting against the NCBI non-redundant (nr), Swiss-Prot, Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Finally, 19,522, 15,691, 14,459, and 10,914 unigenes were identified correspondingly. There were 22,158 differentially expressed genes (DEGs) identified between two stages. Compared with the cyprid stage, 8241 unigenes were down-regulated and 13,917 unigenes were up-regulated at the adult stage. The neuroactive ligand-receptor interaction pathway (ko04080) was significantly enriched by KEGG enrichment analysis of the DEGs, suggesting that it possibly involved in larval settlement. Potential functions of three conserved allatostatin neuropeptide-receptor pairs and two light-sensitive opsin proteins were further characterized, indicating that they might regulate attachment and metamorphosis at cyprid stage. These results provided a deeper insight into the molecular mechanisms underlying larval settlement of barnacles. |
| URL标识 | 查看原文 |
| WOS关键词 | DIEL VERTICAL MIGRATION ; SEX PEPTIDE RECEPTOR ; BALANUS-AMPHITRITE ; JUVENILE-HORMONE ; RNA-SEQ ; FUNCTIONAL-CHARACTERIZATION ; GREGARIOUS SETTLEMENT ; RHODNIUS-PROLIXUS ; METHYL FARNESOATE ; ACORN BARNACLES |
| 语种 | 英语 |
| WOS记录号 | WOS:000416811300027 |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/5270]  |
| 专题 | 深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组 |
| 通讯作者 | He, Lisheng(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China); He, Lisheng |
| 作者单位 | 1.Shenzhen Nobel Sci & Technol Serv Co Ltd, Shenzhen 440305, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Dept Life Sci, Sanya 572000, Peoples R China 3.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jin,Lan, Yi,Zhang, Gen,et al. Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(11). |
| APA | Sun, Jin.,Lan, Yi.,Zhang, Gen.,Yan, Guoyong.,Huang, Jiaomei.,...&Zeng, Cong.(2017).Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(11). |
| MLA | Sun, Jin,et al."Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.11(2017). |
入库方式： OAI收割
来源：深海科学与工程研究所
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