Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops
文献类型：期刊论文
| 作者 | Deng, Yunjin4; Zhao, Chao2,4; Wang, Xingna1 ; Li, Qiuzhe4; Huang, Yifan2,3,4; Liu, Bin2,4
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| 刊名 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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| 出版日期 | 2014-09-01 |
| 卷号 | 24期号:9页码:1280-1290 |
| 关键词 | Microbial Consortium Cellulolytic Bacterium Hot Springs Cellulase Ethanol |
| DOI | 10.4014/jmb.1401.01022 |
| 文献子类 | Article |
| 英文摘要 | In order to obtain the cellulolytic bacterial consortia, sediments from Great Basin hot springs (Nevada, USA) were sampled and enriched with cellulosic biomass as the sole carbon source. The bacterial composition of the resulting anaerobic ethanol-producing celluloytic bacterial consortium, named SV79, was analyzed. With methods of the full-length 16S rRNA library-based analysis and denaturing gradient gel electrophoresis, 21 bacteria belonging to eight genera were detected from this consortium. Clones with closest relation to the genera Acetivibrio, Clostridium, Cellulosilyticum, Ruminococcus, and Sporomusa were predominant. The cellulase activities and ethanol productions of consortium SV79 using different agricultural residues (sugarcane bagasse and spent mushroom substrate) and energy crops (Spartina anglica, Miscanthus floridulus, and Pennisetum sinese Roxb) were studied. During cultivation, consortium SV79 produced the maximum filter paper activity (FPase, 9.41 U/ml), carboxymethylcellulase activity (CMCase, 6.35 U/ml), and xylanase activity (4.28 U/ml) with sugarcane bagasse, spent mushroom substrate, and S. anglica, respectively. The ethanol production using M. floridulus as substrate was up to 2.63 mM ethanol/g using gas chromatography analysis. It has high potential to be a new candidate for producing ethanol with cellulosic biomass under anoxic conditions in natural environments. |
| WOS关键词 | SP-NOV. ; LIGNOCELLULOSIC BIOMASS ; THERMOPHILIC MICROORGANISMS ; CLOSTRIDIUM-THERMOCELLUM ; BIOETHANOL PRODUCTION ; DEGRADING BACTERIA ; HUMAN FECES ; GEN.-NOV. ; PRETREATMENT ; BAGASSE |
| 语种 | 英语 |
| WOS记录号 | WOS:000342394900016 |
| 源URL | [http://124.16.218.2:8080/handle/183446/767]  |
| 专题 | 科学技术处 |
| 作者单位 | 1.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China 2.Natl Engn Res Ctr Juncao, Fuzhou 350002, Fujian, Peoples R China 3.Fujian Agr & Forestry Univ, Coll Anim Sci, Fuzhou 350002, Fujian, Peoples R China 4.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Yunjin,Zhao, Chao,Wang, Xingna,et al. Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2014,24(9):1280-1290. |
| APA | Deng, Yunjin,Zhao, Chao,Wang, Xingna,Li, Qiuzhe,Huang, Yifan,&Liu, Bin.(2014).Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,24(9),1280-1290. |
| MLA | Deng, Yunjin,et al."Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 24.9(2014):1280-1290. |
入库方式： OAI收割
来源：深海科学与工程研究所
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