A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems
文献类型：期刊论文
| 作者 | Xu, J; Yang, BC; Tian, HZ; Guan, YF |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 384期号:7-8页码:1590-1593 |
| 关键词 | fluorescence detector windowless flow cell light-emitting diode capillary HPLC flow injection |
| 通讯作者 | 关亚风 |
| 产权排序 | 1;1 |
| 英文摘要 | a miniaturized fluorescence detector utilizing a three-dimensional windowless flow cell has been constructed and evaluated. the inlet and outlet liquid channels are collinear and are located in the same plane as the excitation paths, while the optical fiber used to collect the emission light is perpendicular to this plane. the straightforward arrangement of the flow path minimizes band dispersion and eliminates bubble formation or accumulation inside the cell. the use of high-brightness light-emitting diodes (leds) as the excitation source and a miniaturized metal package photomultiplier tube (pmt) results in a compact and sensitive fluorescence detector. the detection limit obtained from the system for fluorescein isothiocyanate (fitc) in flow injection mode is 2.6 nmol/l. the analysis of riboflavin and fitc by packed capillary liquid chromatography is demonstrated. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | light-emitting-diode ; wave-guide ; fluorometric detection ; optical-fiber ; electrophoresis ; injection ; separation ; sample |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000236502600019 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97643]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, J,Yang, BC,Tian, HZ,et al. A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems[J]. analytical and bioanalytical chemistry,2006,384(7-8):1590-1593. |
| APA | Xu, J,Yang, BC,Tian, HZ,&Guan, YF.(2006).A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems.analytical and bioanalytical chemistry,384(7-8),1590-1593. |
| MLA | Xu, J,et al."A windowless flow cell-based miniaturized fluorescence detector for capillary flow systems".analytical and bioanalytical chemistry 384.7-8(2006):1590-1593. |
入库方式： OAI收割
来源：大连化学物理研究所
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