Preparation and application of monolithic columns with narrow immobilized pH gradients
文献类型：期刊论文
| 作者 | Zhu, Guijie; Yang, Chun; Zhang, Lihua; Liang, Zhen; Zhang, Weibing; Zhang, Yukui |
| 刊名 | talanta
 |
| 出版日期 | 2006-08-15 |
| 卷号 | 70期号:1页码:2-6 |
| 关键词 | monolithic immobilized pH gradient column preparation narrow pH gradient |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | monolith with immobilized ph gradient (m-ipg) is a novel separation matrix for amphoteric substances, such as peptides and proteins. to improve the properties of the newly designed column, efforts were made to optimize the preparation procedure, including the concentrations of the monomers, glycidyl methacrylate (gma) and ethylene glycol dimethacrylate (edma), as well as the kinds of porogens, which led to a monolith with improved permeability, uniformity and continuity. in addition, different diamines, including ethylenediamine, 1,3-diaminopropane, 1,4-diaminobutane and 1,5-diaminopentane, were subjected to aminate the polymer, and the last two exhibited excellent reactivity with epoxy on the polymer surface, which could obviously make the immobilization of ph gradient facilitated. under the optimal conditions, a simple method to prepare m-ipg column with narrow ph gradient was developed with commercial carrier ampholytes (cas) solution. such columns were applied into the analysis of proteins and peptides, and showed the improved resolution compared to the traditional one with a wide ph distribution. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary electrochromatography ; carrier ampholytes ; stationary phases ; electrophoresis ; state |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000239709400002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97681]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Guijie,Yang, Chun,Zhang, Lihua,et al. Preparation and application of monolithic columns with narrow immobilized pH gradients[J]. talanta,2006,70(1):2-6. |
| APA | Zhu, Guijie,Yang, Chun,Zhang, Lihua,Liang, Zhen,Zhang, Weibing,&Zhang, Yukui.(2006).Preparation and application of monolithic columns with narrow immobilized pH gradients.talanta,70(1),2-6. |
| MLA | Zhu, Guijie,et al."Preparation and application of monolithic columns with narrow immobilized pH gradients".talanta 70.1(2006):2-6. |
入库方式： OAI收割
来源：大连化学物理研究所
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