Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
文献类型：期刊论文
| 作者 | Long, Zhicheng; Liu, Dayu; Ye, Nannan; Qin, Jianhua; Lin, Bingcheng |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 27期号:24页码:4927-4934 |
| 关键词 | filtration microchip electrophoresis nanoporous membrane preconcentration |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | microfluidic devices integrating membrane-based sample preparation with electrophoretic separation are demonstrated. these multilayer devices consist of 10 nm pore diameter membranes sandwiched between two layers of pdms substrates with embedded microchannels. because of the membrane isolation, material exchange between two fluidic layers can be precisely controlled by applied voltages. more importantly, since only small molecules can pass through the nanopores, the integrated membrane can serve as a filter or a concentrator prior to microchip electrophoresis under different design and operation modes. as a filter, they can be used for separation and selective injection of small analytes from sample matrix. this has been effectively applied in rapid determination of reduced glutathione in human plasma and red blood cells without any off-chip deproteinization procedure. alternatively, in the concentrator mode, they can be used for online purification and preconcentration of macromolecules, which was illustrated by removing primers and preconcentrating the product dna from a pcr product mixture. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | ionization mass-spectrometry ; capillary-electrophoresis ; microfluidic devices ; postcolumn reactor ; protein digestion ; pcr amplification ; dna analysis ; separation ; systems ; chip |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000243304100008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98077]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Zhicheng,Liu, Dayu,Ye, Nannan,et al. Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis[J]. electrophoresis,2006,27(24):4927-4934. |
| APA | Long, Zhicheng,Liu, Dayu,Ye, Nannan,Qin, Jianhua,&Lin, Bingcheng.(2006).Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis.electrophoresis,27(24),4927-4934. |
| MLA | Long, Zhicheng,et al."Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis".electrophoresis 27.24(2006):4927-4934. |
入库方式： OAI收割
来源：大连化学物理研究所
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