Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis
文献类型：期刊论文
| 作者 | Liu, Xiaojun; Liang, Aiye; Shen, Zheng; Liu, Xin; Zhang, Yu; Dai, Zhongpeng; Xiong, Bohui; Lin, Bingcheng |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 27期号:24页码:5128-5131 |
| 关键词 | drug-plasma protein interactions frontal analysis microchip electrophoresis |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | we developed a simple, rapid, and sensitive two-injector microchip electrophoresis frontal analysis (mce-fa) method for studying drug-plasma protein interactions. in this method, large volumes of a reference sample and drug-plasma protein mixture were simultaneously introduced into the respective sections of the microchannel through the separated injectors and then electrophoresed. since the reference sample did not meet with the interacting species during migration, it could be used as an external standard. the interaction between heparin and hsa was quantitatively characterized as a model system. the binding constant was found to be (1.53 +/- 0.01) x 10(4) m-1. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | affinity capillary-electrophoresis ; induced fluorescence detection ; amyloid p component ; zone-electrophoresis ; binding ; heparin ; dna ; methodology ; peptide ; assay |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000243304100030 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98081]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaojun,Liang, Aiye,Shen, Zheng,et al. Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis[J]. electrophoresis,2006,27(24):5128-5131. |
| APA | Liu, Xiaojun.,Liang, Aiye.,Shen, Zheng.,Liu, Xin.,Zhang, Yu.,...&Lin, Bingcheng.(2006).Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis.electrophoresis,27(24),5128-5131. |
| MLA | Liu, Xiaojun,et al."Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis".electrophoresis 27.24(2006):5128-5131. |
入库方式： OAI收割
来源：大连化学物理研究所
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