Isotachophoresis preconcentration integrated microfluidic chip for highly sensitive genotyping of the hepatitis B virus
文献类型：期刊论文
| 作者 | Liu, Dayu; Shi, Ming; Huang, Huaiqing; Long, Zhicheng; Zhou, Xiaomian; Qin, Jianhua; Lin, Bingcheng |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2006-11-21 |
| 卷号 | 844期号:1页码:32-38 |
| 关键词 | microfluidic chip sample preconcentration isotachophoresis hepatitis virus B genotyping |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | the genotyping of hepatitis b virus (hbv) has become recently a valuable tool not only for epidemiological reasons but also for the clinical practice. conventional methods for hbv genotyping typically include amplification of the target dna sequences with a two-round nested pcr followed by separation of the amplified fragments by gel electrophoresis. a microfluidic chip that couples isotachophoresis (itp) preconcentration and zone electrophoresis (ze) separation may provide great advantages for sensitive, rapid and cost-effective clinical analysis. in this study, an hbv genotyping method with only one amplification round was developed by the application of the itp-ze chip. all the analysis steps of the itp-ze separation including sample injection, stacking and separation were performed continuously, controlled by sequential high-voltage switching. a 2.1 cm sample plug was preconcentrated between discontinuous buffers in itp process, followed by ze separation. sensitivity enhancement was obtained through the increase of sample loading volume. the average lod value of the itp-ze microfluidic chip was determined to be 0.0021 pg/mu l. in a large-scale hbv genotyping test, single round pcr products were analyzed by itp-ze microfluidic chip, and the results were compared with that of the conventional method. among the 200 cases studied, the classification rate obtained with microfluidic chip was 93%, which was 6% higher than that obtained with the conventional method. method with itp-ze chip analysis provides hbv genotyping information in reduced pcr amplification time with higher detection rate when compared with conventional method. this method holds great potential for extrapolation to the abundance of similar molecular biology-based techniques in clinical diagnosis. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | capillary-electrophoresis ; separation ; infection ; sample ; pcr ; fragments ; microchip ; devices ; risk |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000242328300005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98083]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Dept Biotechnol, Dalian 116023, Peoples R China 3.Guangzhou First Municipal Peoples Hosp, Dept Clin Lab, Guangzhou Med Coll, Guangzhou 510500, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Dayu,Shi, Ming,Huang, Huaiqing,et al. Isotachophoresis preconcentration integrated microfluidic chip for highly sensitive genotyping of the hepatitis B virus[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2006,844(1):32-38. |
| APA | Liu, Dayu.,Shi, Ming.,Huang, Huaiqing.,Long, Zhicheng.,Zhou, Xiaomian.,...&Lin, Bingcheng.(2006).Isotachophoresis preconcentration integrated microfluidic chip for highly sensitive genotyping of the hepatitis B virus.journal of chromatography b-analytical technologies in the biomedical and life sciences,844(1),32-38. |
| MLA | Liu, Dayu,et al."Isotachophoresis preconcentration integrated microfluidic chip for highly sensitive genotyping of the hepatitis B virus".journal of chromatography b-analytical technologies in the biomedical and life sciences 844.1(2006):32-38. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。