Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus
文献类型：期刊论文
| 作者 | Feng, B.; Chen, Y.; Zhao, C.; Zhao, X.; Bai, X.; Du, Y. |
| 刊名 | plant physiology and biochemistry
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 44期号:10页码:596-603 |
| 关键词 | oligochitosan Ser/Thr protein kinase PAL TMV resistance transgenic tobacco |
| 产权排序 | 1;1 |
| 通讯作者 | 冯斌 |
| 英文摘要 | oligochitosan induces defense responses to pathogenic microbes in a wide variety of plants by acting as an elicitor. in the present study, mrna differential display was used to investigate oligochitosan-induced transcriptional activation of defense-related genes. accordingly, a novel ser/thr protein kinase gene was isolated and designated as oligochitosan-induced protein kinase (oipk). molecular cloning showed that oipk contains six introns interrupted by seven exons. the open reading frame (orf) of the gene is 1848 bp, which encodes a putative protein of 615 amino acids with the predicted molecular mass of 70.96 kda and a pi of 6.32. a plant oipk antisense expression vector was constructed and transformed into tobacco by agrobacterium tumefaciens. decreased phenylalanine ammonia-lyase (pal, ec 4.3.1.5) activity and decreased resistance to tobacco mosaic virus (tmv) were observed in transgenic tobacco. rt-pcr analysis revealed that oipk was expressed at high levels after oligochitosan induction in wild-type tobacco, but not in transgenic tobacco. these results indicated that oipk is involved in the signal pathway of oligochitosan-induced resistance in tobacco. (c) 2006 elsevier masson sas. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | plant sciences |
| 研究领域[WOS] | plant sciences |
| 关键词[WOS] | high-affinity binding ; n-acetylchitooligosaccharide elicitor ; cultured rice cells ; systemic acquired-resistance ; phenylalanine ammonia-lyase ; disease resistance ; defense responses ; plasma-membrane ; signal-transduction ; chitin fragments |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000242710500011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98331]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Liaoning Normal Univ, Coll Life Sci, Dalian 116029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, B.,Chen, Y.,Zhao, C.,et al. Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus[J]. plant physiology and biochemistry,2006,44(10):596-603. |
| APA | Feng, B.,Chen, Y.,Zhao, C.,Zhao, X.,Bai, X.,&Du, Y..(2006).Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus.plant physiology and biochemistry,44(10),596-603. |
| MLA | Feng, B.,et al."Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus".plant physiology and biochemistry 44.10(2006):596-603. |
入库方式： OAI收割
来源：大连化学物理研究所
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