Advances in chromatographic techniques and methods in shotgun proteome analysis
文献类型：期刊论文
| 作者 | Ye, Mingliang; Jiang, Xiaogang; Feng, Shun; Tian, Ruijun; Zou, Hanfa |
| 刊名 | trac-trends in analytical chemistry
 |
| 出版日期 | 2007 |
| 卷号 | 26期号:1页码:80-84 |
| 关键词 | automated proteome-analysis system multidimensional separation one-dimensional separation separation technique shotgun proteomics |
| ISSN号 | 0165-9936 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | shotgun proteomics is a high-throughput approach to proteome analysis whereby the protein mixture is digested and the peptides generated are separated by capillary liquid chromatography and sequenced by tandem mass spectrometry (ms). due to the huge number of peptide species, separation prior to ms analysis plays an important role in shotgun proteomics. overall sensitivity, dynamic range, throughput and general effectiveness of shotgun proteomic analysis largely depend on how well the peptide mixture is separated. in recent years, new separation techniques have been applied successfully to proteome analysis and have dramatically improved protein identification. we briefly review the recent development of chromatographic techniques and methods in shotgun proteome analysis, including the following three aspects: one-dimensional separation; multidimensional separation; and, automated proteome-analysis systems. (c) 2006 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; performance liquid-chromatography ; free-flow electrophoresis ; human plasma proteome ; coded affinity tags ; multidimensional chromatography ; capillary columns ; nanoelectrospray ionization ; identification technology ; monolithic capillary |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000244120400021 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98433]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Mingliang,Jiang, Xiaogang,Feng, Shun,et al. Advances in chromatographic techniques and methods in shotgun proteome analysis[J]. trac-trends in analytical chemistry,2007,26(1):80-84. |
| APA | Ye, Mingliang,Jiang, Xiaogang,Feng, Shun,Tian, Ruijun,&Zou, Hanfa.(2007).Advances in chromatographic techniques and methods in shotgun proteome analysis.trac-trends in analytical chemistry,26(1),80-84. |
| MLA | Ye, Mingliang,et al."Advances in chromatographic techniques and methods in shotgun proteome analysis".trac-trends in analytical chemistry 26.1(2007):80-84. |
入库方式： OAI收割
来源：大连化学物理研究所
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