Fe3+ immobilized metal affinity chromatography with silica monolithic capillary column for phosphoproteome analysis
文献类型：期刊论文
| 作者 | Feng, Shun; Pan, Chensong; Jiang, Xiaogang; Xu, Songyu; Zhou, Houjiang; Ye, Mingliang; Zou, Hanfa |
| 刊名 | proteomics
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 7期号:3页码:351-360 |
| 关键词 | immobilized metal affinity chromatography miniaturization phosphoproteome analysis silica monolithic column |
| ISSN号 | 1615-9853 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | immobilized metal affinity chromatography (imac) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. miniaturization of imac column is essential for the analysis of a small amount of sample. nanoscale imac column vas prepared by chemical m6dification of silica monolith with iminodiacetic acid (ida) followed by the immobilization of fe3+ ion inside the capillary. it was demonstrated that fe3+-ida silica monolithic imac capillary column could specifically capture the phosphopeptides from tryptic digest of (x-casein with analysis by maldi-tof ms. the silica monolithic imac capillary column was manually coupled with nanoflow rplc/nanospray esi mass spectrometer (mu rplc-nanoesi ms) for phosphoproteome analysis. the system was validated by analysis of standard phosphoproteins and then it was applied to the analysis of protein phosphorylation in mouse liver lysate. besides ms/ms spectra, ms/ms/ms spectra were also collected for neutral loss peak. after database search and manual validation with conservative criteria, 29 singly phosphorylated peptides were identified by analyzing a tryptic digest of only 12 mu g mouse liver lysate. the results demonstrated that the silica monolithic imac capillary column coupled with grplc-nanoesi ms was very suitable for the phosphoproteome analysis of minute sample. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | ionization mass-spectrometry ; protein-phosphorylation sites ; ion affinity ; liquid-chromatography ; proteomic analysis ; phase-separation ; maldi-ms ; identification ; online ; phosphopeptides |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000244271500002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98459]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China 2.Xinjiang Univ, Coll Chem & Chem Engn, Urumqi, Xinjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Shun,Pan, Chensong,Jiang, Xiaogang,et al. Fe3+ immobilized metal affinity chromatography with silica monolithic capillary column for phosphoproteome analysis[J]. proteomics,2007,7(3):351-360. |
| APA | Feng, Shun.,Pan, Chensong.,Jiang, Xiaogang.,Xu, Songyu.,Zhou, Houjiang.,...&Zou, Hanfa.(2007).Fe3+ immobilized metal affinity chromatography with silica monolithic capillary column for phosphoproteome analysis.proteomics,7(3),351-360. |
| MLA | Feng, Shun,et al."Fe3+ immobilized metal affinity chromatography with silica monolithic capillary column for phosphoproteome analysis".proteomics 7.3(2007):351-360. |
入库方式： OAI收割
来源：大连化学物理研究所
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