Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds
文献类型：期刊论文
| 作者 | Kang, Xihai; Xie, Yubing; Powell, Heather M.; Lee, L. James; Belury, Martha A.; Lannutti, John J.; Kniss, Douglas A. |
| 刊名 | biomaterials
 |
| 出版日期 | 2007 |
| 卷号 | 28期号:3页码:450-458 |
| 关键词 | adipose tissue engineering stem cell electrospinning polycaprolactone three-dimensional cell culture morphogenesis |
| 产权排序 | 7;2 |
| 通讯作者 | douglas a. kniss |
| 英文摘要 | a mechanistic understanding of adipose tissue differentiation is critical for the treatment and prevention of obesity and type 2 diabetes. conventional in vitro models of adipogenesis are preadipocytes or freshly isolated adipocytes grown in two-dimensional (2d) cultures. optimal results using in vitro tissue culture models can be expected only when adipocyte models closely resemble adipose tissue in vivo. thus the design of an in vitro three-dimensional (3d) model which faithfully mimics the in vivo environment is needed to effectively study adipogenesis. pluripotent embryonic stem (es) cells are a self-renewing cell type that can readily be differentiated into adipocytes. in this study, a 3d culture system was developed to mimic the geometry of adipose tissue in vivo. murine es cells were seeded into electrospun polycaprolactone scaffolds and differentiated into adipocytes in situ by hormone induction as demonstrated using a battery of gene and protein expression markers along with the accumulation of neutral lipid droplets. insulin-responsive akt phosphorylation, and beta-adrenergic stimulation of cyclic amp synthesis were demonstrated in es cell-derived adipocytes. morphologically, es cell-derived adipocytes resembled native fat cells by scanning electron and phase contrast microscopy. this tissue engineered es cell-matrix model has potential uses in drug screening and other therapeutic developments. (c) 2006 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | engineering, biomedical ; materials science, biomaterials |
| 研究领域[WOS] | engineering ; materials science |
| 关键词[WOS] | fibroblast-growth-factor ; adipose-tissue ; in-vitro ; adipocyte differentiation ; vivo ; expression ; biology ; model ; morphogenesis ; organization |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000242960900009 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98469]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Ohio State Univ, Coll Med, Dept Obstet & Gynecol, Lab Perinatal Res, Columbus, OH 43210 USA 2.Ohio State Univ, Nanoscale Sci & Engn Ctr Affordable Nanoengn Poly, Columbus, OH 43210 USA 3.Ohio State Univ, Dept Biomed Engn, Columbus, OH 43210 USA 4.Ohio State Univ, Dept Mat Sci & Engn, Columbus, OH 43210 USA 5.Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA 6.Ohio State Univ, Dept Human Nutr, Columbus, OH 43210 USA 7.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kang, Xihai,Xie, Yubing,Powell, Heather M.,et al. Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds[J]. biomaterials,2007,28(3):450-458. |
| APA | Kang, Xihai.,Xie, Yubing.,Powell, Heather M..,Lee, L. James.,Belury, Martha A..,...&Kniss, Douglas A..(2007).Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds.biomaterials,28(3),450-458. |
| MLA | Kang, Xihai,et al."Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds".biomaterials 28.3(2007):450-458. |
入库方式： OAI收割
来源：大连化学物理研究所
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