Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues
文献类型：期刊论文
| 作者 | Jiang, Xiaogang; Jiang, Xinning; Feng, Shun; Tian, Ruijun; Ye, Mingliang; Zou, Hanfa |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 6期号:3页码:1038-1047 |
| 关键词 | formalin-fixed tissue protein extraction shotgun proteome tandem mass spectrometry |
| ISSN号 | 1535-3893 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | there are vast archives of formalin-fixed tissues spanning many conceivable conditions such as different diseases, time courses, and different treatment and allowing acquisition of the necessary numbers of samples to carry out biomarker discovery study. however, the conventional protein analysis approach is not applicable for the analysis of proteins in the formalin-fixed tissue because the formalin fixation process resulted in the cross-linking of proteins, and thus, intact proteins cannot be efficiently extracted. in this study, several protocols were investigated to extract proteins from formalin-fixed mouse liver tissue for shotgun proteome analysis. it was found that incubation of tissue in a lysis buffer containing 6 m guanidine hydrochloride at high temperature led to the highest protein yield and the largest number of proteins identified. the peptides and proteins identified from formalin-fixed tissue were first comprehensively compared with those identified from frozen-fresh tissue. it was found that a majority of peptides identified from fixed tissue were unmodified and proteome coverage for the analysis of fixed tissue was not obviously compromised by the formalin fixation process. valuable proteome information could be obtained by shotgun proteome analysis of formalin-fixed tissue, which presents a new approach for disease biomarker discovery. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | difference gel-electrophoresis ; tandem mass-spectrometry ; candidate cancer biomarkers ; paraffin-embedded tissue ; ionization emitter ; yeast proteome ; identification ; ms/ms ; immunohistochemistry ; separation |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000244638400012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98493]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog B&A Ctr, Dalian 116023, Peoples R China 2.Suzhou Univ, Sch Med, Suzhou 215007, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Xiaogang,Jiang, Xinning,Feng, Shun,et al. Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues[J]. journal of proteome research,2007,6(3):1038-1047. |
| APA | Jiang, Xiaogang,Jiang, Xinning,Feng, Shun,Tian, Ruijun,Ye, Mingliang,&Zou, Hanfa.(2007).Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues.journal of proteome research,6(3),1038-1047. |
| MLA | Jiang, Xiaogang,et al."Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues".journal of proteome research 6.3(2007):1038-1047. |
入库方式： OAI收割
来源：大连化学物理研究所
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