Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes
文献类型：期刊论文
| 作者 | Li, Xin; Xu, Songyun; Pan, Chensong; Zhou, Houjiang; Jiang, Xiaogang; Mang, Yu; Ye, Mingliang; Zou, Hanfa |
| 刊名 | journal of separation science
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 30期号:6页码:930-943 |
| 关键词 | carbon nanotubes human plasma liquid chromatography mass spectrometry peptidome |
| ISSN号 | 1615-9306 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | human plasma contains a complex matrix of proteolytically derived peptides (plasma peptidome) that may provide a correlate of biological events occurring in the entire organism. analyzing these peptides from a small amount of serum/plasma is difficult due to the complexity of the sample and the low levels of these peptides. here, we describe a novel peptidome analysis approach using multiwalled carbon nanotubes (mwcnts) as an alternative adsorbent to capture endogenous peptides from human plasma. harvested peptides were analyzed by using liquid chromatography-mass spectrometry as a means of detecting and assessing the adsorbed molecules. the improved sensitivity and resolution obtained by using liquid chromatography-mass spectrometry allowed detection of 2521 peptide features (m/z 300-1800 range) in about 50 mu l of plasma. 374 unique peptides were identified with high confidence by two-dimensional liquid chromatography system coupled to a nano-spray ionization linear ion trap-mass spectrometer. high recovery of bsa digest peptides enriched with mwcnts, in both standard buffer and high abundance protein solution, was observed. comparative studies showed that mwcnts were superior to c18 and c8 for the capture of the smaller peptides. this approach could hold promise of routine plasma peptidome analysis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; solid-phase extraction ; liquid-chromatography ; human serum ; proteomic analysis ; remove albumin ; proteins ; capture ; markers ; column |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000246348000019 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98545]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xin,Xu, Songyun,Pan, Chensong,et al. Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes[J]. journal of separation science,2007,30(6):930-943. |
| APA | Li, Xin.,Xu, Songyun.,Pan, Chensong.,Zhou, Houjiang.,Jiang, Xiaogang.,...&Zou, Hanfa.(2007).Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes.journal of separation science,30(6),930-943. |
| MLA | Li, Xin,et al."Enrichment of peptides from plasma for peptidome analysis using multiwalled carbon nanotubes".journal of separation science 30.6(2007):930-943. |
入库方式： OAI收割
来源：大连化学物理研究所
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