Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines
文献类型：期刊论文
| 作者 | Su, Xingye; Hu, Lianghai; Kong, Liang; Lei, Xiaoyuan; Zou, Hanfa |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2007-06-22 |
| 卷号 | 1154期号:1-2页码:132-137 |
| 关键词 | affinity chromatography biological fingerprinting analysis immobilized DNA stationary phase traditional Chinese medicines |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a stationary phase for high performance affinity chromatography with immobilization of dna onto silica gel was prepared and characterized. the effect of the ionic strength, concentration of mg2+, edta and ch3 cn in the mobile phase on the retention of alkaloids were investigated. with this stationary phase, biological fingerprinting analysis of traditional chinese medicines (tcms) coptis chinensis franch and rheum palmatum l. was performed with both one-dimensional (1-d) and two-dimensional (2-d) chromatography. the 1-d chromatography was performed with isocratic and gradient elution and 2-d chromatography was developed with immobilized dna column combined with silica monolithic ods column. it was found that 7 compounds in coptis chinensis franch including berberine, palmatine and jatrorrhizine, 14 compounds in rheum palmatum l. including aloe-emodin, rhein, emodin, chrysophannol-8-o-glucophranoside and physionl-8-o-glucophranoside were active in binding to the immobilized dna. (c) 2007 elsevier b.v all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | l-rna aptamer ; bioactive compounds ; angelica-sinensis ; in-vitro ; binding ; target ; purification ; spectrometry ; selectivity ; separation |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000247419300016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98615]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Xingye,Hu, Lianghai,Kong, Liang,et al. Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines[J]. journal of chromatography a,2007,1154(1-2):132-137. |
| APA | Su, Xingye,Hu, Lianghai,Kong, Liang,Lei, Xiaoyuan,&Zou, Hanfa.(2007).Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines.journal of chromatography a,1154(1-2),132-137. |
| MLA | Su, Xingye,et al."Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines".journal of chromatography a 1154.1-2(2007):132-137. |
入库方式： OAI收割
来源：大连化学物理研究所
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