Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis
文献类型：期刊论文
| 作者 | Zhou, Houjiang; Tian, Ruijun; Ye, Mingliang; Xu, Songyun; Feng, Shun; Pan, Chensong; Jiang, Xiaogang; Li, Xin; Zou, Hanfa |
| 刊名 | electrophoresis
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 28期号:13页码:2201-2215 |
| 关键词 | MALDI-TOF MS nano-LC MS/MS phosphopeptides phosphoproteome analysis zirconium dioxide nanoparticles |
| ISSN号 | 0173-0835 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | large-scale characterization of phosphoproteins requires highly specific methods for the purification of phosphopeptides because of the low abundance of phosphoproteins and substoichiometry of phosphorylation. a phosphopeptide enrichment method using zro2 nanoparticles is presented. the high specificity of this approach was demonstrated by the isolation of phosphopeptides from the digests of model phosphoproteins. the strong affinity of zro2 nanoparticles to phosphopeptides enables the specific enrichment of phosphopeptides from a complex peptide mixture in which the abundance of phosphopeptides is two orders of magnitude lower than that of nonphosphopeptides. superior selectivity of zro2 nanoparticles for the enrichment of phosphorylated peptides than that of conventional immobilized metal affinity chromatography was observed. femtomole phosphopeptides from digestion products could be enriched by zro2 nanoparticles and can be well detected by maldi mass spectrometric analysis. zro2 nanoparticles were further applied to selectively isolate phosphopeptides from the tryptic digestion of mouse liver lysate for phosphoproteome analysis by nanoliter lc ms/ms (nano-lc-ms/ms) and ms/ms/ms. a total of 248 defining phosphorylation sites and 140 phosphorylated peptides were identified by manual validation using a series of rigid criteria. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | mass-spectrometry ; affinity-chromatography ; phosphorylated proteins ; tyrosine phosphorylation ; posttranslational modifications ; surface-properties ; proteomic analysis ; ms analysis ; lc-ms/ms ; identification |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000248190400010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98655]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Houjiang,Tian, Ruijun,Ye, Mingliang,et al. Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis[J]. electrophoresis,2007,28(13):2201-2215. |
| APA | Zhou, Houjiang.,Tian, Ruijun.,Ye, Mingliang.,Xu, Songyun.,Feng, Shun.,...&Zou, Hanfa.(2007).Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis.electrophoresis,28(13),2201-2215. |
| MLA | Zhou, Houjiang,et al."Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis".electrophoresis 28.13(2007):2201-2215. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。