Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device
文献类型：期刊论文
| 作者 | Ye, Nannan; Qin, Jianhua; Shi, Weiwei; Lin, Bingcheng |
| 刊名 | electrophoresis
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 28期号:7页码:1146-1153 |
| 关键词 | apoptosis doxorubicin human hepatocellular carcinoma cells microfluidic device |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | apoptosis has now established its importance in numerous areas of biology and is recently receiving great attention as an important topic related to the development of diseases. in this work, an integrated microfluidic device was developed to characterize doxorubicin-induced apoptosis in human hepatocellular carcinoma (hepg2) cells. a continuous concentration gradient of stimulator (doxorubicin) was generated in the upstream network and used to perfuse downstream cultured hepg2 cells. the appropriate fluorescent dyes were introduced into cells from the inlets connected to the cell culture chambers, allowing one to distinguish apoptotic cells from nonapoptotic or necrotic cells. the resultant fluorescence of cellular population was monitored and quantified with single-cell resolution to infer the apoptosis process being studied. the feasibility of studying apoptosis was demonstrated by measuring several apoptotic events, including morphological alterations, plasma membrane phosphatidylserine externalization, and mitochondrial membrane potential collapse. this microfluidic device, integrating the cell culture, stimulation, staining, and washing steps into a single device, can simultaneously generate a number of experimental conditions and investigate multiple parameters relating stimulation to apoptosis. it offers a unique platform to characterize various cellular responses in a high-throughput fashion, which is otherwise impossible with conventional methods. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | total analysis systems ; differentiation ; generation ; gradients ; hallmarks ; death ; flow |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000245682000015 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98693]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device[J]. electrophoresis,2007,28(7):1146-1153. |
| APA | Ye, Nannan,Qin, Jianhua,Shi, Weiwei,&Lin, Bingcheng.(2007).Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device.electrophoresis,28(7),1146-1153. |
| MLA | Ye, Nannan,et al."Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device".electrophoresis 28.7(2007):1146-1153. |
入库方式： OAI收割
来源：大连化学物理研究所
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