Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA
文献类型：期刊论文
| 作者 | Zhong, Runtao; Liu, Dayu; Yu, Linfen; Ye, Nannan; Dai, Zhongpeng; Oin, Jianhua; Lin, Bingcheng |
| 刊名 | electrophoresis
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 28期号:16页码:2920-2926 |
| 关键词 | DNA purification microfabrication packed column solid-phase extraction two-weir structure |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | microchip-based packed column spe of dna was performed using the microfabricated two-weir structure within a microchannel. we developed two methods to fabricate the two-weir structured glass chips: a "two-side etching/alignment" method and a simplified "one-side/two-step etching" method. the former rnethod required a straightforward alignment step, while the latter approach comprised a simplified wet etching process using paraffin wax as the temporary protective layer. both methods were convenient and rapid as compared to the previous approaches. through a reversibly sealed bead-introduction channel, beads can be fed into or out of the chip columns, thus enabling refreshment of the packing materials. using the proposed chip columns, highly efficient,lambda-dna extractions (average recovery > 80%) were performed with good chip-to-chip reproducibility (rsd < 10%). the on-chip spe procedure was completed within 15 min at the flow rate of 3 mu l/min and the bulk of the loaded dna was eluted within a small volume of similar to 8 mu l. application of the microchip-based packed columns was demonstrated by purifying pcr-amplifiable genomic dna from human hepatocellular carcinoma (hepg2) cells and human whole blood samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | polymerase-chain-reaction ; microfluidic chips ; sample preparation ; nucleic-acids ; microchip ; purification ; system ; device ; preconcentration ; amplification |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000249286500018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98699]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Runtao,Liu, Dayu,Yu, Linfen,et al. Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA[J]. electrophoresis,2007,28(16):2920-2926. |
| APA | Zhong, Runtao.,Liu, Dayu.,Yu, Linfen.,Ye, Nannan.,Dai, Zhongpeng.,...&Lin, Bingcheng.(2007).Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA.electrophoresis,28(16),2920-2926. |
| MLA | Zhong, Runtao,et al."Fabrication of two-weir structure-based packed columns for on-chip solid-phase extraction of DNA".electrophoresis 28.16(2007):2920-2926. |
入库方式： OAI收割
来源：大连化学物理研究所
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