Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis
文献类型：期刊论文
| 作者 | Shen, Zheng; Liu, Xiaojun; Zhou, Xiaomian; Liang, Aiye; Wu, Dapeng; Yu, Linfen; Dai, Zhongpeng; Qin, Jianhua; Lin, Bingcheng |
| 刊名 | journal of separation science
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 30期号:10页码:1544-1548 |
| 关键词 | capillary array electrophoresis DNA interaction microfluidic chip netropsin |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | microfabricated capillary array electrophoresis (mu-cae) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(cccctataccgc) center dot d(gcggtatagggg). esi-ms was used to provide an independent verification of the microchip electrophoresis derived data. simultaneous parallel quantitative assay of multiple samples was performed in a single run (< 50 s) on the self-developed mu-cae device. the binding constant and stoichiometry calculated from scatchard plot were (2.88 +/- 0.23) x 10(5) m-1 and 1:1, respectively. the values showed a good quantitative agreement with the results determined by esi-ms and those using other methods reported in the literature. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | ionization mass-spectrometry ; drug-dna interactions ; zone-electrophoresis ; analysis systems ; affinity ; sequence ; chip ; selectivity ; complexes |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000248240000018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98701]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Guangzhou Med Coll, Dept Clin Lab, Guangzhou Municipal Peoples Hosp 1, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Zheng,Liu, Xiaojun,Zhou, Xiaomian,et al. Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis[J]. journal of separation science,2007,30(10):1544-1548. |
| APA | Shen, Zheng.,Liu, Xiaojun.,Zhou, Xiaomian.,Liang, Aiye.,Wu, Dapeng.,...&Lin, Bingcheng.(2007).Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis.journal of separation science,30(10),1544-1548. |
| MLA | Shen, Zheng,et al."Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis".journal of separation science 30.10(2007):1544-1548. |
入库方式： OAI收割
来源：大连化学物理研究所
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