Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis
文献类型：期刊论文
| 作者 | Wang, Fangjun; Dong, Jing; Jiang, Xiaogang; Ye, Mingliang; Zou, Hanfa |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 79期号:17页码:6599-6606 |
| ISSN号 | 0003-2700 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a 150 mu m internal diameter capillary monolithic column with a strong cation-exchange stationary phase was prepared by direct in situ polymerization of ethylene glycol methacrylate phosphate and bisacrylamide in a trinary porogenic solvent consisting dimethylsulfoxide, dodecanol, and n,n '-dimethylformamide. this phosphate monolithic column exhibits higher dynamic binding capacity, faster kinetic adsorption of peptides, and more than 10 times higher permeability than the column packed with commercially available strong cation-exchange particles. it was applied as a trap column in a nanoflow liquid chromatography-tandem mass spectrometry system for automated sample injection and online multidimensional separation. it was observed that the sample could be loaded at a flow rate as high as 40 mu l/min with a back pressure of similar to 1300 psi and without compromising the separation efficiency. because of its good orthogonality to the reversed phase separation mechanism, the phosphate monolithic trap column was coupled with a reversed-phase column for online multidimensional separation of 19 mu g of the tryptic digest of yeast proteins. a total of 1522 distinct proteins were identified from 5608 unique peptides (total of 54 780 peptides) at the false positive rate only 0.46%. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; organic polymer monoliths ; tandem mass-spectrometry ; human plasma proteome ; stationary phases ; multidimensional chromatography ; nanoelectrospray ionization ; identification technology ; surface-chemistry ; membrane-proteins |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000249242900029 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98731]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Dong, Jing,Jiang, Xiaogang,et al. Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis[J]. analytical chemistry,2007,79(17):6599-6606. |
| APA | Wang, Fangjun,Dong, Jing,Jiang, Xiaogang,Ye, Mingliang,&Zou, Hanfa.(2007).Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis.analytical chemistry,79(17),6599-6606. |
| MLA | Wang, Fangjun,et al."Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis".analytical chemistry 79.17(2007):6599-6606. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。