Selective extraction of peptides in acidic human plasma by porous silica nanoparticles for peptidome analysis with 2-D LC-MS/MS
文献类型：期刊论文
| 作者 | Tian, Ruijun; Ye, Mingliang; Hu, Lianghai; Li, Xin; Zou, Hanfa |
| 刊名 | journal of separation science
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 30期号:14页码:2204-2209 |
| 关键词 | human plasma peptidome mass spectrometry mesoporous silica 2-D nano-LC peptidases |
| ISSN号 | 1615-9306 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | in this study, an improved method for human plasma peptidome analysis including selective porous silica nanoparticles (mcm-41) extraction and subsequent online 2-d nano-lc-ms/ms analysis was established. enhanced enrichment efficiency for the mcm-41 extraction was obtained by adjusting the ph of the plasma sample to 2.5. a total of 1680 unique peptides were identified in the plasma sample obtained from one healthy donor, which is nearly twice the amount identified from the native state of the plasma sample. the hydrophobic property, molecular weight (mw), and pl distribution of the identified peptides at ph 2.5 and native state of the plasma sample were systematically investigated and compared. furthermore, many unusual cleaved peptides from plasma proteins (e.g., hsa) were observed at ph 2.5, which clearly show a ladder pattern. the cleavage patterns for all of the identified peptides at ph 2.5 were summarized, and chymosin and cathepsin d were confirmed as the possible peptidases responsible for the change of cleavage pattern in peptide profiling. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; serum peptidome ; proteome ; particles |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000249800300005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98741]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Ruijun,Ye, Mingliang,Hu, Lianghai,et al. Selective extraction of peptides in acidic human plasma by porous silica nanoparticles for peptidome analysis with 2-D LC-MS/MS[J]. journal of separation science,2007,30(14):2204-2209. |
| APA | Tian, Ruijun,Ye, Mingliang,Hu, Lianghai,Li, Xin,&Zou, Hanfa.(2007).Selective extraction of peptides in acidic human plasma by porous silica nanoparticles for peptidome analysis with 2-D LC-MS/MS.journal of separation science,30(14),2204-2209. |
| MLA | Tian, Ruijun,et al."Selective extraction of peptides in acidic human plasma by porous silica nanoparticles for peptidome analysis with 2-D LC-MS/MS".journal of separation science 30.14(2007):2204-2209. |
入库方式： OAI收割
来源：大连化学物理研究所
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