Parallel microfluidic networks for studying cellular response to chemical modulation
文献类型：期刊论文
| 作者 | Liu, Dayu; Wang, Lihui; Zhong, Runtao; Li, Bowei; Ye, Nannan; Liu, Xin; Lin, Bingcheng |
| 刊名 | journal of biotechnology
 |
| 出版日期 | 2007-09-15 |
| 卷号 | 131期号:3页码:286-292 |
| 关键词 | microfluidic chip gradient concentration glutathione drug resistance |
| 产权排序 | 3;1 |
| 通讯作者 | 刘大渔 ; 林炳承 |
| 英文摘要 | a microfluidic chip featuring parallel gradient-generating networks etched on glass plate was designed and fabricated. the dam and weir structures were fabricated to facilitate cell positioning and seeding, respectively. the microchip contains five gradient generators and 30 cell chambers where the resulted concentration gradients of drugs are delivered to stimulate the on-chip cultured cells. this microfluidics exploits the advantage of lab-on-a-chip technology by integrating the generation of drug concentration gradients and a series of cell operations including seeding, culture, stimulation and staining into a chip. steady parallel concentration gradients were generated by flowing two fluids in each network. the microchip described above was applied in studying the role of reduced glutathione (gsh) in mcf-7 cells' chemotherapy sensitivity. the parental breast cancer cell line, mcf-7 and the derived adriamycin resistant cell line mcf-7(adm) were treated with concentration gradients of arsenic trioxide (ato) and n-acetyl cysteine (nac) for gsh modulation, followed by exposure to adriamycin. the intracellular gsh level and cell viability were assessed by fluorescence image analysis. gsh levels of both cell lines were down-regulated upon ato treatment and up-regulated upon nac treatment. for both cell lines, suppression of intracellular gsh by treatment with ato has been shown to increase chemotherapy sensitivity; conversely, elevation of intracellular gsh by treatment with nac leads to increased drug resistance. the results indicated that high intracellular gsh level has negative effect on chemotherapy sensitivity, while depletion of cellular gsh may serve as an effective way to improve chemotherapy sensitivity. the integrated microfluidic chip is able to perform multiparametric pharmacological profiling with easy operation, thus, holds great potential for extrapolation to the high-content drug screening. (c) 2007 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | drug-resistance ; glutathione ; induction ; cells |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000250031300010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/98891]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Improve Med Instruments Co Ltd, Dept Biomed Engn Ctr, Guangzhou 510370, Guangdong, Peoples R China 2.Dalian Med Univ, Dept Pathol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Dayu,Wang, Lihui,Zhong, Runtao,et al. Parallel microfluidic networks for studying cellular response to chemical modulation[J]. journal of biotechnology,2007,131(3):286-292. |
| APA | Liu, Dayu.,Wang, Lihui.,Zhong, Runtao.,Li, Bowei.,Ye, Nannan.,...&Lin, Bingcheng.(2007).Parallel microfluidic networks for studying cellular response to chemical modulation.journal of biotechnology,131(3),286-292. |
| MLA | Liu, Dayu,et al."Parallel microfluidic networks for studying cellular response to chemical modulation".journal of biotechnology 131.3(2007):286-292. |
入库方式： OAI收割
来源：大连化学物理研究所
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