Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic
文献类型：期刊论文
| 作者 | Schneider, Olha1; Simic, Nebojsa2; Aachmann, Finn Lillelund1; Rueckert, Christian3; Kristiansen, Kare Andre1; Kalinowski, Joern3; Jiang, Yi4; Wang, Lisong5 ; Jiang, Cheng-Lin4; Lale, Rahmi1
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| 刊名 | FRONTIERS IN MICROBIOLOGY
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| 出版日期 | 2018-12-19 |
| 卷号 | 9页码:12 |
| 关键词 | new Streptomyces sp. from lichen antibacterial activity genome mining new thiopeptide antibiotic berninamycins |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2018.03139 |
| 通讯作者 | Zotchev, Sergey B.(sergey.zotchev@univie.ac.at) |
| 英文摘要 | Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against Bacillus subtilis. The draft genome sequence of this isolate strain revealed 18 putative biosynthetic gene clusters (BGCs) for secondary metabolites, which is an unusually low number compared to a typical streptomycete. Inactivation of a (antibiotic dehydrogenase-encoding gene from the BGC presumed to govern biosynthesis of a thiopeptide resulted in the loss of bioactivity. Using comparative HPLC analysis, two peaks in the chromatogram were identified in the extract from the wild-type strain, which were missing in the extract from the mutant. The compounds corresponding to the identified peaks were purified, and structure of one compound was elucidated using NMR. The compound, designated geninthiocin B, showed high similarity to several 35-membered macrocyclic thiopeptides geninthiocin, Val-geninthiocin and berninamycin A. Bioinformatics analysis of the geninthiocin B BGC revealed its close homology to that of berninamycins. |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000453853900001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/64661]  |
| 专题 | 中国科学院昆明植物研究所 |
| 通讯作者 | Zotchev, Sergey B. |
| 作者单位 | 1.Norwegian Univ Sci & Technol, Dept Biotechnol & Food Sci, Trondheim, Norway 2.Norwegian Univ Sci & Technol, Dept Chem, Trondheim, Norway 3.Bielefeld Univ, Ctr Biotechnol, Bielefeld, Germany 4.Yunnan Univ, Yunnan Inst Microbiol, Kunming, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China 6.Univ Vienna, Dept Pharmacognosy, Vienna, Austria |
| 推荐引用方式 GB/T 7714 | Schneider, Olha,Simic, Nebojsa,Aachmann, Finn Lillelund,et al. Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic[J]. FRONTIERS IN MICROBIOLOGY,2018,9:12. |
| APA | Schneider, Olha.,Simic, Nebojsa.,Aachmann, Finn Lillelund.,Rueckert, Christian.,Kristiansen, Kare Andre.,...&Zotchev, Sergey B..(2018).Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic.FRONTIERS IN MICROBIOLOGY,9,12. |
| MLA | Schneider, Olha,et al."Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic".FRONTIERS IN MICROBIOLOGY 9(2018):12. |
入库方式： OAI收割
来源：昆明植物研究所
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