Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens
文献类型：期刊论文
| 作者 | Wang, Yi; Geng, Changan; Yuan, Xiaolong; Hua, Mei; Tian, Fenghua; Li, Changtian |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2018 |
| 卷号 | 13期号:7页码:e0199110 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0199110 |
| 通讯作者 | Li, Changtian(lct@jlau.edu.cn) |
| 英文摘要 | Usnic acid is a unique polyketide produced by lichens. To characterize usnic acid biosynthesis, the transcriptome of the usnic-acid-producing lichen-forming fungus Nephromopsis pallescens was sequenced using Illumina NextSeq technology. Seven complete non-reducing polyketide synthase genes and nine highly-reducing polyketide synthase genes were obtained through transcriptome analysis. Gene expression results obtained by qPCR and usnic acid detection with LCMS-IT-TOF showed that Nppks7 is probably involved in usnic acid biosynthesis in N. pallescens. Nppks7 is a non-reducing polyketide synthase with a MeT domain that also possesses beta-ketoacyl-ACP synthase, acyl transferase, product template, acyl carrier protein, C-methyltransferase, and Claisen cyclase domains. Phylogenetic analysis shows that Nppks7 and other polyketide synthases from lichens form a unique monophyletic Glade. Taken together, our data indicate that Nppks7 is a novel PKS in N. pallescens that is likely involved in usnic acid biosynthesis. |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000439022400009 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/64708]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Yi,Geng, Changan,Yuan, Xiaolong,et al. Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens[J]. PLOS ONE,2018,13(7):e0199110. |
| APA | Wang, Yi,Geng, Changan,Yuan, Xiaolong,Hua, Mei,Tian, Fenghua,&Li, Changtian.(2018).Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens.PLOS ONE,13(7),e0199110. |
| MLA | Wang, Yi,et al."Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens".PLOS ONE 13.7(2018):e0199110. |
入库方式： OAI收割
来源：昆明植物研究所
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