Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.
文献类型：期刊论文
| 作者 | Li, Shun; Huang, Jun-Chao
|
| 刊名 | Natural products and bioprospecting
 |
| 出版日期 | 2018 |
| 卷号 | 8期号:5页码:397-403 |
| ISSN号 | 2192-2195 |
| 英文摘要 | Astaxanthin is a value-added ketocarotenoid with great potential in nutraceutical and pharmaceutical industries. Genetic engineering of heterologous hosts for astaxanthin production has attracted great attention. In this study, we assessed some key factors, including codon usage of the expressed genes, types of promoters, bacterial strains, and culture media, for engineered Escherichia coli to produce astaxanthin. The effect of codon usage was shown to be related to the types of promoters. E. coli DH5alpha was superior to other strains for astaxanthin production. Different culture media greatly affected the contents and yields of astaxanthin in engineered E. coli. When the expression cassette containing GadE promoter and its driving genes, HpCHY and CrBKT, was inserted into the plasmid pACCAR16DeltacrtX and expressed in E. coli DH5alpha, the engineered strain was able to produce 4.30±0.28mg/g dry cell weight (DCW) or 24.16±2.03mg/L of astaxanthin, which was a sevenfold or 40-fold increase over the initial production of 0.62±0.03mg/g DCW or 0.61±0.05mg/L. |
| 语种 | 英语 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/64786]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Li, Shun,Huang, Jun-Chao. Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.[J]. Natural products and bioprospecting,2018,8(5):397-403. |
| APA | Li, Shun,&Huang, Jun-Chao.(2018).Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin..Natural products and bioprospecting,8(5),397-403. |
| MLA | Li, Shun,et al."Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.".Natural products and bioprospecting 8.5(2018):397-403. |
入库方式： OAI收割
来源：昆明植物研究所
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