Microbe-focused glycan array screening platform
文献类型：期刊论文
| 作者 | Geissner, Andreas1,2,9; Reinhardt, Anika1,2,10; Rademacher, Christoph1,2; Johannssen, Timo1,2; Monteiro, Joao3,4; Lepenies, Bernd1,2,3,4; Thepaut, Michel5,6,7; Fieschi, Franck5,6,7; Mrazkova, Jana8; Wimmerova, Michaela8 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2019-02-05 |
| 卷号 | 116期号:6页码:1958-1967 |
| 关键词 | glycan arrays microbial antigens immune receptors antiglycan antibodies bacterial lectins |
| ISSN号 | 0027-8424 |
| 英文摘要 | Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans. |
| 资助项目 | Max Planck Society ; Zukunftsfonds Berlin ; European Regional Development Fund Grant GlyCarDiag ; European Research Council Advanced Grant AUTOHEPARIN ; German Federal Ministry for Education and Research[0315447] ; Korber Foundation ; Studienstiftung des Deutschen Volkes ; German Research Foundation[RA1944/2-1] ; Deutsche Forschungsgemeinschaft[LE 2498/6-1] ; European Union's Horizon 2020 Research and Innovation Program (Marie Sklodowska-Curie Grant)[642870] ; project CEITEC 2020 from the Ministry of Education, Youth and Sports of the Czech Republic[LQ1601] ; FRISBI[ANR-10-INSB-05-02] ; GRAL Grant[ANR-10-LABX-49-01] ; French Agence Nationale de la Recheche (ANR)[ANR- 15-IDEX-02] |
| 语种 | 英语 |
| 资助机构 | Max Planck Society ; Zukunftsfonds Berlin ; European Regional Development Fund Grant GlyCarDiag ; European Research Council Advanced Grant AUTOHEPARIN ; German Federal Ministry for Education and Research ; Korber Foundation ; Studienstiftung des Deutschen Volkes ; German Research Foundation ; Deutsche Forschungsgemeinschaft ; European Union's Horizon 2020 Research and Innovation Program (Marie Sklodowska-Curie Grant) ; project CEITEC 2020 from the Ministry of Education, Youth and Sports of the Czech Republic ; FRISBI ; GRAL Grant ; French Agence Nationale de la Recheche (ANR) |
| 源URL | [http://ir.kib.ac.cn/handle/151853/64217]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Seeberger, Peter H. |
| 作者单位 | 1.Max Planck Inst Colloids & Interfaces, Dept Biomol Syst, D-14424 Potsdam, Germany 2.Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany 3.Univ Vet Med Hannover, Immunol Unit, D-30559 Hannover, Germany 4.Univ Vet Med Hannover, Res Ctr Emerging Infect & Zoonoses, D-30559 Hannover, Germany 5.Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France 6.CNRS, Inst Biol Struct, F-38044 Grenoble, France 7.Commissariat Energie Atom & Energies Alternat, Inst Biol Struct, F-38044 Grenoble, France 8.Masaryk Univ, Cent European Inst Technol, Brno 62500, Czech Republic 9.Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada 10.Kyoto Univ, Dept Life Sci Frontiers, Ctr iPS Cell Res & Applicat, Kyoto 6068507, Japan |
| 推荐引用方式 GB/T 7714 | Geissner, Andreas,Reinhardt, Anika,Rademacher, Christoph,et al. Microbe-focused glycan array screening platform[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(6):1958-1967. |
| APA | Geissner, Andreas.,Reinhardt, Anika.,Rademacher, Christoph.,Johannssen, Timo.,Monteiro, Joao.,...&Seeberger, Peter H..(2019).Microbe-focused glycan array screening platform.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(6),1958-1967. |
| MLA | Geissner, Andreas,et al."Microbe-focused glycan array screening platform".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.6(2019):1958-1967. |
入库方式： OAI收割
来源：昆明植物研究所
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