Novel dual-color drug screening model for GLUT4 translocation in adipocytes
文献类型：期刊论文
| 作者 | Lu, Yanting1,3; Ma, Xiuli1,3; Kong, Qinghua1; Xu, Yuhui1; Hu, Jing1,3; Wang, Fang1,3; Qin, Wanying1,3; Wang, Libin2; Xiong, Wenyong1,2
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| 刊名 | MOLECULAR AND CELLULAR PROBES
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| 出版日期 | 2019-02-01 |
| 卷号 | 43页码:6-12 |
| 关键词 | GLUT4 pHluorin Screening Glucose uptake |
| ISSN号 | 0890-8508 |
| 英文摘要 | Insulin-responsive glucose transporter type 4 (GLUT4) translocation plays a major role in controlling glucose uptake in adipose tissue and muscle, maintaining homeostasis and preventing hyperglycemia. Screening for chemicals enhancing GLUT4 translocation is an approach for identifying hits of drug development for type 2 diabetes. Here we developed a novel functional dual-color probe, pHluorin-GLUT4-mOrange2, and constructed 3T3-L1 adipocytes based screening system to simply and efficiently screen new compounds stimulating GLUT4 translocation. Based on this system, we successfully identified a few hits facilitating GLUT4 translocation. In conclusion, we developed an easy-to-apply dual color GLUT4 probe to monitor GLUT4 translocation in insulin-responsive cells, which could be alternatively employed to high-throughput screen compounds regulating GLUT4 translocation and glucose uptake, even to dissect GLTU4 approaching, docking and fusion with the plasma membrane (PM), and to reveal relevant molecular mechanisms involved in these steps as expected. |
| 资助项目 | Yunnan Provincial Science and Technology Department of China[2017FA044] ; Yunnan Provincial Science and Technology Department of China[2013HA023] ; National Natural Science Foundations of China[81560474] ; Light of West China Program of the Chinese Academy of Science[2014-91] |
| 语种 | 英语 |
| 资助机构 | Yunnan Provincial Science and Technology Department of China ; National Natural Science Foundations of China ; Light of West China Program of the Chinese Academy of Science |
| 源URL | [http://ir.kib.ac.cn/handle/151853/64244]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Wang, Libin; Xiong, Wenyong |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Ningxia Med Univ, Dept Beijing Natl Biochip Res Ctr Subctr Ningxia, Gen Hosp, Yinchuan 750004, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Yanting,Ma, Xiuli,Kong, Qinghua,et al. Novel dual-color drug screening model for GLUT4 translocation in adipocytes[J]. MOLECULAR AND CELLULAR PROBES,2019,43:6-12. |
| APA | Lu, Yanting.,Ma, Xiuli.,Kong, Qinghua.,Xu, Yuhui.,Hu, Jing.,...&Xiong, Wenyong.(2019).Novel dual-color drug screening model for GLUT4 translocation in adipocytes.MOLECULAR AND CELLULAR PROBES,43,6-12. |
| MLA | Lu, Yanting,et al."Novel dual-color drug screening model for GLUT4 translocation in adipocytes".MOLECULAR AND CELLULAR PROBES 43(2019):6-12. |
入库方式： OAI收割
来源：昆明植物研究所
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