Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis
文献类型：期刊论文
| 作者 | Wang, Fangjun; Jiang, Xiaogang; Feng, Shun; Tian, Ruijun; Jiang, Xinning; Han, Guanghui; Liu, Hongwei; Ye, Mingliang; Zou, Hanfa |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2007-11-09 |
| 卷号 | 1171期号:1-2页码:56-62 |
| 关键词 | mu HPLC-MS/MS shotgun proteome analysis automated sample injection SCX trap column void volume insensitive multidimensional separation |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (mu hplc-ms/ms). in order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 mu l on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (scx) trap column was investigated, it was found the void volume hardly affects the separation performance by using scx trap column. thus, a fully automated sample injection system using scx trap column and ten-port switching valve was established for efficient shotgun proteome analysis. in this system, a nanoflow switching valve and a microtee were used to connect the scx trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. (c) 2007 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; performance liquid-chromatography ; monolith capillary column ; shotgun proteomics ; ionization emitter ; identification ; peptides ; system ; phase ; ms |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000250793400008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99043]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograp R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Jiang, Xiaogang,Feng, Shun,et al. Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis[J]. journal of chromatography a,2007,1171(1-2):56-62. |
| APA | Wang, Fangjun.,Jiang, Xiaogang.,Feng, Shun.,Tian, Ruijun.,Jiang, Xinning.,...&Zou, Hanfa.(2007).Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis.journal of chromatography a,1171(1-2),56-62. |
| MLA | Wang, Fangjun,et al."Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis".journal of chromatography a 1171.1-2(2007):56-62. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。