Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like
文献类型：期刊论文
| 作者 | Li, Jian1; Ma, Lan1; Zhang, Shuting1,2; Zuo, Cailian1; Song, Na1; Zhu, Shusheng3; Wu, Jinsong1 |
| 刊名 | PLANTA
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 249期号:4页码:1229-1237 |
| 关键词 | 1-and 3-year-old Panax notoginseng Dammarene-type triterpene saponins RNA-seq Transgenic tobacco |
| ISSN号 | 0032-0935 |
| 英文摘要 | Main conclusionTranscriptome analysis revealed high expression of saponin biosynthetic genes may account for highly accumulated saponins in 3-year-oldPanax notoginsengroots andDSandCYP716A47-likewere functionally verified by transgenic tobacco.Panax notoginseng is a well-known traditional medical herb that contains bioactive compounds known as saponins. Three major dammarene-type triterpene saponins including R1, Rb1, and Rg1 were found to be highly accumulated in the roots of 3-year-old plants when compared to those of 1-year-old plants. However, the underlying cellular mechanism is poorly understood. In this study, transcriptome analysis revealed that most genes involved in saponin biosynthesis in P. notoginseng roots augmented during their growth periods. The analysis of the KEGG pathway indicated that the primary metabolism, cell growth, and differentiation were less active in the roots of 3-year-old plant; however, secondary metabolisms were enhanced, thus providing molecular evidence for the harvesting of P. notoginseng roots in the 3rd year of growth. Furthermore, the functional role of DS and CYP716A47-like, two of the candidate genes involved in saponin biosynthesis isolated from P. notoginseng, were verified via overexpression in cultivated tobacco. Approximately, 0.325 mu gg(-1) of dammarenediol-II and 0.320 mu gg(-1) of protopanaxadiol were recorded in the dry leaves of transgenic tobacco overexpressed with DS and both DS and CYP716A47-like, respectively. This study provides insights into the molecular mechanisms for saponin accumulation in P. notoginseng roots during its growth period and paves a promising way to produce dammarenediol-II and protopanaxadiol via transgenic techniques. |
| 语种 | 英语 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/65179]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 通讯作者 | Wu, Jinsong |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Lanhei Rd 132, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Key Lab Agrobiodivers & Pest Management, Educ Minist China, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jian,Ma, Lan,Zhang, Shuting,et al. Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like[J]. PLANTA,2019,249(4):1229-1237. |
| APA | Li, Jian.,Ma, Lan.,Zhang, Shuting.,Zuo, Cailian.,Song, Na.,...&Wu, Jinsong.(2019).Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like.PLANTA,249(4),1229-1237. |
| MLA | Li, Jian,et al."Transcriptome analysis of 1-and 3-year-old Panax notoginseng roots and functional characterization of saponin biosynthetic genes DS and CYP716A47-like".PLANTA 249.4(2019):1229-1237. |
入库方式： OAI收割
来源：昆明植物研究所
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