Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain
文献类型：期刊论文
| 作者 | Hu, Y-H.1; Dang, W.1,2; Liu, C-S.1,2; Sun, L.1; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 50期号:1页码:97-103 |
| 关键词 | Copper Edwardsiella Tarda Luxs Virulence |
| ISSN号 | 0266-8254 |
| DOI | 10.1111/j.1472-765X.2009.02761.x |
| 文献子类 | Article |
| 英文摘要 | Aim:; Aim: To investigate the effect of copper on the virulence of Edwardsiella tarda. Methods and Results: The pathogenic Edw. tarda strain TX5 was cultured under copper-stressed conditions and examined for any potential alteration in capacities that are associated with pathogenicity. The results showed that compared to untreated TX5, Cu-treated TX5 exhibits reduced planktonic and biofilm growth, an impaired ability to adhere to host mucus, modulation of host immune response, and dissemination in host blood and liver. Consistent with these observations, the overall bacterial virulence of Cu-treated TX5 is significantly attenuated. SDS-PAGE analyses of whole cell protein production showed that Cu-treated TX5 differs from the untreated TX5 in its production of at least one protein. Quantitative real time reverse transcriptase PCR analyses showed that copper treatment decreased the expression of virulence-associated genes encoding components of the type III and type VI secretion systems, the Eth haemolysin system, and the LuxS/AI-2 quorum-sensing system. Conclusions: Prolonged exposure to copper has multiple effects on TX5 and results in significant attenuation of bacterial virulence. Significance and Impact of the Study: The results of this study demonstrate that copper treatment has a broad and profound effect on the virulence-associated capacities of TX5, which is exerted at least in part at the transcription level. These findings provide new insights to the antimicrobial mechanism of copper. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000272582600016 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1604]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Y-H.,Dang, W.,Liu, C-S.,et al. Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain[J]. LETTERS IN APPLIED MICROBIOLOGY,2010,50(1):97-103. |
| APA | Hu, Y-H.,Dang, W.,Liu, C-S.,Sun, L.,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain.LETTERS IN APPLIED MICROBIOLOGY,50(1),97-103. |
| MLA | Hu, Y-H.,et al."Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strain".LETTERS IN APPLIED MICROBIOLOGY 50.1(2010):97-103. |
入库方式： OAI收割
来源：海洋研究所
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