Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis
文献类型：期刊论文
| 作者 | Li, F.1,2; Wang, L.1 ; Zhang, H.1; Zheng, P.1; Zhao, J.1; Qiu, L.1; Zhang, Y.1; Song, L.1; Wang, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
|
| 刊名 | INTERNATIONAL JOURNAL OF IMMUNOGENETICS
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 37期号:6页码:499-508 |
| 关键词 | Nf-kappa-b Innate Immune-response Mosquito Aedes-aegypti Spot Syndrome Virus Tremor Disease Transcription Factors Pathogen Infection Dna-binding Drosophila Activation |
| ISSN号 | 1744-3121 |
| DOI | 10.1111/j.1744-313X.2010.00954.x |
| 文献子类 | Article |
| 英文摘要 | P>NF-kappa B is a B-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. In the present study, a novel NF-kappa B-like transcription factor Relish was cloned from Chinese mitten crab Eriocheir sinensis (designated as EsRelish) by rapid amplification of cDNA ends (RACE) technique based on expressed sequence tag (EST). The full-length cDNA of EsRelish was of 5034 bp, consisting of a 5' untranslated region (UTR) of 57 bp, a 3' UTR of 1335 bp with two mRNA instability motifs (ATTTA), a polyadenylation signal sequence (AATAAA) and a poly (A) tail, and an open reading frame (ORF) of 3645 bp encoding a polypeptide of 1214 amino acids with a calculated molecular mass of 134.8 kDa and a theoretical isoelectric point of 5.26. There were a typical Rel homology domain (RHD), two nuclear localization signal (NLS) sequences (KR), an inhibitor kappa B (I kappa B)-like domain with six ankyrin repeats, a PEST region and a death domain in the deduced amino acid sequence of EsRelish. Conserved domain, higher similarity with other Rel/NF-kappa Bs and phylogenetic analysis suggested that EsRelish was a member of the NF-kappa B family. Quantitative real-time RT-PCR was employed to detect the mRNA transcripts of EsRelish in different tissues and its temporal expression in hemocytes of E. sinensis challenged with Pichia methanolica and Listonella anguillarum. The EsRelish mRNA was found to be constitutively expressed in a wide range of tissues. It could be mainly detected in the hemocytes, gonad and hepatopancreas, and less degree in the gill, muscle and heart. The expression level of EsRelish mRNA in hemocytes was up-regulated from at 3, 6, 9 and 12 h after P. methanolica challenge. In L. anguillarum challenge, it was up-regulated at 9, 12 and 24 h. The results collectively indicated that EsRelish was potentially involved in the immune response against fungus and bacteria.; P>NF-kappa B is a B-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. In the present study, a novel NF-kappa B-like transcription factor Relish was cloned from Chinese mitten crab Eriocheir sinensis (designated as EsRelish) by rapid amplification of cDNA ends (RACE) technique based on expressed sequence tag (EST). The full-length cDNA of EsRelish was of 5034 bp, consisting of a 5' untranslated region (UTR) of 57 bp, a 3' UTR of 1335 bp with two mRNA instability motifs (ATTTA), a polyadenylation signal sequence (AATAAA) and a poly (A) tail, and an open reading frame (ORF) of 3645 bp encoding a polypeptide of 1214 amino acids with a calculated molecular mass of 134.8 kDa and a theoretical isoelectric point of 5.26. There were a typical Rel homology domain (RHD), two nuclear localization signal (NLS) sequences (KR), an inhibitor kappa B (I kappa B)-like domain with six ankyrin repeats, a PEST region and a death domain in the deduced amino acid sequence of EsRelish. Conserved domain, higher similarity with other Rel/NF-kappa Bs and phylogenetic analysis suggested that EsRelish was a member of the NF-kappa B family. Quantitative real-time RT-PCR was employed to detect the mRNA transcripts of EsRelish in different tissues and its temporal expression in hemocytes of E. sinensis challenged with Pichia methanolica and Listonella anguillarum. The EsRelish mRNA was found to be constitutively expressed in a wide range of tissues. It could be mainly detected in the hemocytes, gonad and hepatopancreas, and less degree in the gill, muscle and heart. The expression level of EsRelish mRNA in hemocytes was up-regulated from at 3, 6, 9 and 12 h after P. methanolica challenge. In L. anguillarum challenge, it was up-regulated at 9, 12 and 24 h. The results collectively indicated that EsRelish was potentially involved in the immune response against fungus and bacteria. |
| 学科主题 | Genetics & Heredity ; Immunology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000283373500010 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1628]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Wang, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, F.,Wang, L.,Zhang, H.,et al. Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis[J]. INTERNATIONAL JOURNAL OF IMMUNOGENETICS,2010,37(6):499-508. |
| APA | Li, F..,Wang, L..,Zhang, H..,Zheng, P..,Zhao, J..,...&Wang, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis.INTERNATIONAL JOURNAL OF IMMUNOGENETICS,37(6),499-508. |
| MLA | Li, F.,et al."Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis".INTERNATIONAL JOURNAL OF IMMUNOGENETICS 37.6(2010):499-508. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。