Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)
文献类型：期刊论文
| 作者 | Liu, Qing Hua1; Chen, Ya Kun1,2; Xiao, Zhi Zhong1; Li, Jun1; Xu, Shi Hong1; Shi, Xue Hui3; Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2010-08-16 |
| 卷号 | 41期号:9页码:e89-e95 |
| 关键词 | Red Seabream Sperm Cryopreservation Storage Time Cryoprotectant |
| ISSN号 | 1355-557X |
| DOI | 10.1111/j.1365-2109.2009.02466.x |
| 文献子类 | Article |
| 英文摘要 | This study examined the effects of storage time and cryoprotectant concentrations on the post-thaw sperm of red seabream, Pagrus major. Sperm treated with 12%, 15%, 18% and 21% DMSO were cryopreserved for 10, 30, 60 and 360 days, and fertilization and hatching rates were analysed. For all groups, there were no differences in the fertilization rates and hatching rates between sperm cryopreserved for < 60 days and fresh sperm (98.8 +/- 0.8%, 96.4 +/- 1.3%). However, for sperm cryopreserved for 360 days, both fertilization rates (88.6 +/- 3.0% to 7.0 +/- 1.9%) and hatching rates (79.4 +/- 7.2% to 3.3 +/- 0.8%) decreased drastically. Furthermore, the cryoprotectant concentrations affected sperm quality significantly (P < 0.05). When cryopreserved for 360 days, sperm treated with 15% DMSO obtained the best results compared with other concentrations. We suggest that 15% DMSO may be an effective cryoprotectant for long-term sperm cryopreservation of red seabream.; This study examined the effects of storage time and cryoprotectant concentrations on the post-thaw sperm of red seabream, Pagrus major. Sperm treated with 12%, 15%, 18% and 21% DMSO were cryopreserved for 10, 30, 60 and 360 days, and fertilization and hatching rates were analysed. For all groups, there were no differences in the fertilization rates and hatching rates between sperm cryopreserved for < 60 days and fresh sperm (98.8 +/- 0.8%, 96.4 +/- 1.3%). However, for sperm cryopreserved for 360 days, both fertilization rates (88.6 +/- 3.0% to 7.0 +/- 1.9%) and hatching rates (79.4 +/- 7.2% to 3.3 +/- 0.8%) decreased drastically. Furthermore, the cryoprotectant concentrations affected sperm quality significantly (P < 0.05). When cryopreserved for 360 days, sperm treated with 15% DMSO obtained the best results compared with other concentrations. We suggest that 15% DMSO may be an effective cryoprotectant for long-term sperm cryopreservation of red seabream. |
| 学科主题 | Fisheries |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000281059700010 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1648]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Coll Earth Sci, Grad Sch, Beijing, Peoples R China 3.QingdaoAgr Univ, Org Dept, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qing Hua,Chen, Ya Kun,Xiao, Zhi Zhong,et al. Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)[J]. AQUACULTURE RESEARCH,2010,41(9):e89-e95. |
| APA | Liu, Qing Hua.,Chen, Ya Kun.,Xiao, Zhi Zhong.,Li, Jun.,Xu, Shi Hong.,...&Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China.(2010).Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843).AQUACULTURE RESEARCH,41(9),e89-e95. |
| MLA | Liu, Qing Hua,et al."Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)".AQUACULTURE RESEARCH 41.9(2010):e89-e95. |
入库方式： OAI收割
来源：海洋研究所
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