Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)
文献类型：期刊论文
| 作者 | Sun, Jianwei; Liu, Tao ; Gu, Baotai; Jin, Demin; Weng, Manli; Feng, Yanbin; Xu, Pu; Duan, Delin; Wang, Bin; Wang, B, Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
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| 刊名 | EUROPEAN JOURNAL OF PHYCOLOGY
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| 出版日期 | 2006-08-01 |
| 卷号 | 41期号:3页码:329-336 |
| 关键词 | Cssr Dna Fingerprint Microsatellite Porphyra Seaweeds Simple Sequence Repeats (Ssrs) |
| ISSN号 | 0967-0262 |
| DOI | 10.1080/09670260600740906 |
| 文献子类 | Article |
| 英文摘要 | This paper reports the development of SSR markers from EST data and their utilization in germplasm identification of Porphyra. The publicly available EST (expressed sequence tag) sequences of Porphyra were searched from the Internet (www.kazura.or.jp/en/plant/porphyra/EST/). From a total of 20,779 obtained EST sequences, 391 SSRs (simple sequence repeats) were analysed with SSRIT software (www.gramene.org/db/searches/ssrtool). From those, 48 SSR primer-pairs were designed and tested by commonly used SSR reaction conditions using 22 Porphyra DNA samples as templates. Results showed that 41 SSR primer-pairs gave good amplification patterns. These were used to conduct SSR analyses of genetic diversity and variety identification of the 22 Porphyra lines. A dendrogram and the DNA fingerprints of the Porphyra lines were developed based on the obtained SSR data.; This paper reports the development of SSR markers from EST data and their utilization in germplasm identification of Porphyra. The publicly available EST (expressed sequence tag) sequences of Porphyra were searched from the Internet (www.kazura.or.jp/en/plant/porphyra/EST/). From a total of 20,779 obtained EST sequences, 391 SSRs (simple sequence repeats) were analysed with SSRIT software (www.gramene.org/db/searches/ssrtool). From those, 48 SSR primer-pairs were designed and tested by commonly used SSR reaction conditions using 22 Porphyra DNA samples as templates. Results showed that 41 SSR primer-pairs gave good amplification patterns. These were used to conduct SSR analyses of genetic diversity and variety identification of the 22 Porphyra lines. A dendrogram and the DNA fingerprints of the Porphyra lines were developed based on the obtained SSR data. |
| 学科主题 | Plant Sciences ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000240755200007 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1668]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, B, Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Laiyang Agr Coll, Fac Life Sci, Laiyang 265200, Peoples R China 3.Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China 4.Jiangsu Inst Oceanol & Hydrol, Lucknow 226007, Uttar Pradesh, India 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jianwei,Liu, Tao,Gu, Baotai,et al. Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2006,41(3):329-336. |
| APA | Sun, Jianwei.,Liu, Tao.,Gu, Baotai.,Jin, Demin.,Weng, Manli.,...&Wang, B, Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China.(2006).Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta).EUROPEAN JOURNAL OF PHYCOLOGY,41(3),329-336. |
| MLA | Sun, Jianwei,et al."Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)".EUROPEAN JOURNAL OF PHYCOLOGY 41.3(2006):329-336. |
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来源：海洋研究所
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