Identification of vhhP2, a novel genetic marker of Vibrio harveyi, and its application in the quick detection of V. harveyi from animal specimens and environmental samples
文献类型：期刊论文
| 作者 | Sun, K.1,2; Hu, Y. -H.1,2; Zhang, X. -H.3; Bai, F. -F.3; Sun, L.1; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 刊名 | JOURNAL OF APPLIED MICROBIOLOGY
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| 出版日期 | 2009-10-01 |
| 卷号 | 107期号:4页码:1251-1257 |
| ISSN号 | 1364-5072 |
| 关键词 | Genetic Marker Pcr Detection Phylogenetic Vibrio Harveyi |
| DOI | 10.1111/j.1365-2672.2009.04304.x |
| 文献子类 | Article |
| 英文摘要 | Aims:; Aims: To investigate the species-specific prevalence of vhhP2 among Vibrio harveyi isolates and the applicability of vhhP2 in the specific detection of V. harveyi from crude samples of animal and environmental origins. Methods and Results: A gene (vhhP2) encoding an outer membrane protein of unknown function was identified from a pathogenic V. harveyi isolate. vhhP2 is present in 24 V. harveyi strains isolated from different geographical locations but is absent in 24 strains representing 17 different non-V. harveyi species, including V. parahaemolyticus and V. alginolyticus. A simple polymerase chain reaction method for the identification of V. harveyi was developed based on the conserved sequence of vhhP2. This method was demonstrated to be applicable to the quick detection of V. harveyi from crude animal specimens and environmental samples. The specificity of this method was tested by applying it to the examination of two strains of V. campbellii, which is most closely related to V. harveyi. One of the V. campbellii strains was falsely identified as V. harveyi. Conclusions: vhhP2 is ubiquitously present in the V. harveyi species and is absent in most of the non-V. harveyi species; this feature enables vhhP2 to serve as a genetic marker for the rapid identification of V. harveyi. However, this method can not distinguish some V. campbellii strains from V. harveyi. Significance and Impact of the Study: the significance of our study is the identification of a novel gene of V. harveyi and the development of a simple method for the relatively accurate detection of V. harveyi from animal specimens and environmental samples. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000269730300021 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1670]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Ocean Univ China, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, K.,Hu, Y. -H.,Zhang, X. -H.,et al. Identification of vhhP2, a novel genetic marker of Vibrio harveyi, and its application in the quick detection of V. harveyi from animal specimens and environmental samples[J]. JOURNAL OF APPLIED MICROBIOLOGY,2009,107(4):1251-1257. |
| APA | Sun, K.,Hu, Y. -H.,Zhang, X. -H.,Bai, F. -F.,Sun, L.,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2009).Identification of vhhP2, a novel genetic marker of Vibrio harveyi, and its application in the quick detection of V. harveyi from animal specimens and environmental samples.JOURNAL OF APPLIED MICROBIOLOGY,107(4),1251-1257. |
| MLA | Sun, K.,et al."Identification of vhhP2, a novel genetic marker of Vibrio harveyi, and its application in the quick detection of V. harveyi from animal specimens and environmental samples".JOURNAL OF APPLIED MICROBIOLOGY 107.4(2009):1251-1257. |
入库方式： OAI收割
来源：海洋研究所
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