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Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation

文献类型：期刊论文

作者Wang, Jinfeng1,2; Jiang, Peng1; Cui, Yulin1,2; Guan, Xiangyu1; Qin, Song1; Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
刊名PHYCOLOGIA
出版日期2010-07-01
卷号49期号:4页码:355-360
关键词Egfp Genetic Transformation Lacz Porphyra Haitanensis Sv40 Promoter
ISSN号0031-8884
DOI10.2216/09-91.1
文献子类Article
英文摘要Genetic transformation by electroporation of protoplasts is a standard procedure for many plants. However, for the genus Porphyra, the method is not effective because of low viability of protoplasts and is a time-consuming and expensive procedure. Based on the life history of Porphyra, a spore-targeted strategy of genetic transformation was developed, that is, using fresh conchospores of Porphyra haitanensis Chang & Zheng transformed by agitation with glass beads. A SV40 promoter-driven lacZ reporter gene was expressed in conchospores 48 h after the agitation. More transformants were obtained by increasing the agitation time from 10 to 25 s. The maximum number of transformants was more than six out of 1 million agitated conchospores. Transfer of a SV40 promoter-driven egfp gene into conchospores resulted in significant green GFP fluorescence. The expression of lacZ and egfp revealed that this strategy of spore-targeted transformation using glass bead agitation is feasible in P. haitanensis and that the SV40 promoter is effective for monitoring expression of foreign genes in this red algal species.; Genetic transformation by electroporation of protoplasts is a standard procedure for many plants. However, for the genus Porphyra, the method is not effective because of low viability of protoplasts and is a time-consuming and expensive procedure. Based on the life history of Porphyra, a spore-targeted strategy of genetic transformation was developed, that is, using fresh conchospores of Porphyra haitanensis Chang & Zheng transformed by agitation with glass beads. A SV40 promoter-driven lacZ reporter gene was expressed in conchospores 48 h after the agitation. More transformants were obtained by increasing the agitation time from 10 to 25 s. The maximum number of transformants was more than six out of 1 million agitated conchospores. Transfer of a SV40 promoter-driven egfp gene into conchospores resulted in significant green GFP fluorescence. The expression of lacZ and egfp revealed that this strategy of spore-targeted transformation using glass bead agitation is feasible in P. haitanensis and that the SV40 promoter is effective for monitoring expression of foreign genes in this red algal species.
学科主题Plant Sciences ; Marine & Freshwater Biology
URL标识查看原文
语种英语
WOS记录号WOS:000280232300006
公开日期2010-11-18
源URL[http://ir.qdio.ac.cn/handle/337002/1684]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生态与环境科学重点实验室
通讯作者Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Jinfeng,Jiang, Peng,Cui, Yulin,et al. Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation[J]. PHYCOLOGIA,2010,49(4):355-360.
APA Wang, Jinfeng,Jiang, Peng,Cui, Yulin,Guan, Xiangyu,Qin, Song,&Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.(2010).Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation.PHYCOLOGIA,49(4),355-360.
MLA Wang, Jinfeng,et al."Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation".PHYCOLOGIA 49.4(2010):355-360.

入库方式： OAI收割

来源：海洋研究所

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