A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)
文献类型：期刊论文
| 作者 | Wang, LL; Song, LS; Chang, YQ; Xu, W; Ni, DJ; Guo, XM; Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2005-05-13 |
| 卷号 | 36期号:7页码:643-653 |
| 关键词 | Chlamys Farreri Genetic Map Aflp Scallop Aquaculture |
| ISSN号 | 1355-557X |
| DOI | 10.1111/j.1365-2109.2005.01268.x |
| 文献子类 | Article |
| 英文摘要 | Zhikong scallop (Chlamys farreri Jones et Preston 1904) is one of the most important aquaculture species in China. The development of a genetic linkage map would provide a powerful tool for the genetic improvement of this species. Amplified fragment length polymorphism (AFLP) is a PCR-based technique that has proven to be powerful in genome fingerprinting and mapping, and population analysis. Genetic maps of C. farreri were constructed using AFLP markers and a full-sib family with 60 progeny. A total of 503 segregating AFLP markers were obtained, with 472 following the Mendelian segregation ratio of 1:1 and 31 markers showing significant (P< 0.05) segregation distortion. The male map contained 166 informative AFLP markers in 23 linkage groups covering 2468 cM. The average distance between markers was 14.9 cM. The female genetic map consisted of 198 markers in 25 linkage groups spanning 3130 cM with an average inter-marker spacing of 15.8 cM. DNA polymorphisms that segregated in a 3:1 ratio as well as the AFLP markers that were heterozygous in both parents were included to construct combined linkage genetic map. Five shared linkage groups, ranging from 61.1 to 162.5 cM, were identified between the male and female maps, covering 431 cM. Amplified fragment length polymorphism markers appeared to be evenly distributed within the linkage groups. Although preliminary, these maps provide a starting point for the mapping of the functional genes and quantitative trait loci in C. farreri.; Zhikong scallop (Chlamys farreri Jones et Preston 1904) is one of the most important aquaculture species in China. The development of a genetic linkage map would provide a powerful tool for the genetic improvement of this species. Amplified fragment length polymorphism (AFLP) is a PCR-based technique that has proven to be powerful in genome fingerprinting and mapping, and population analysis. Genetic maps of C. farreri were constructed using AFLP markers and a full-sib family with 60 progeny. A total of 503 segregating AFLP markers were obtained, with 472 following the Mendelian segregation ratio of 1:1 and 31 markers showing significant (P< 0.05) segregation distortion. The male map contained 166 informative AFLP markers in 23 linkage groups covering 2468 cM. The average distance between markers was 14.9 cM. The female genetic map consisted of 198 markers in 25 linkage groups spanning 3130 cM with an average inter-marker spacing of 15.8 cM. DNA polymorphisms that segregated in a 3:1 ratio as well as the AFLP markers that were heterozygous in both parents were included to construct combined linkage genetic map. Five shared linkage groups, ranging from 61.1 to 162.5 cM, were identified between the male and female maps, covering 431 cM. Amplified fragment length polymorphism markers appeared to be evenly distributed within the linkage groups. Although preliminary, these maps provide a starting point for the mapping of the functional genes and quantitative trait loci in C. farreri. |
| 学科主题 | Fisheries |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000228866900004 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1686]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Fisheries Coll, Dalian, Peoples R China 4.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ USA |
| 推荐引用方式 GB/T 7714 | Wang, LL,Song, LS,Chang, YQ,et al. A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)[J]. AQUACULTURE RESEARCH,2005,36(7):643-653. |
| APA | Wang, LL.,Song, LS.,Chang, YQ.,Xu, W.,Ni, DJ.,...&Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2005).A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904).AQUACULTURE RESEARCH,36(7),643-653. |
| MLA | Wang, LL,et al."A preliminary genetic map of Zhikong scallop (Chlamys farreri Jones et Preston 1904)".AQUACULTURE RESEARCH 36.7(2005):643-653. |
入库方式： OAI收割
来源：海洋研究所
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