Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain
文献类型：期刊论文
| 作者 | Zhang, Min; Sun, Kun; Sun, Li; Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 刊名 | MICROBIOLOGY-SGM
 |
| 出版日期 | 2008 |
| 卷号 | 154期号:Part 7页码:2060-2069 |
| 关键词 | Controls Biofilm Formation Quorum-sensing System Escherichia-coli Salmonella-typhimurium Vibrio-harveyi Streptococcus-pyogenes Gene-expression Iii Secretion Virulence Metabolism |
| ISSN号 | 1350-0872 |
| 英文摘要 | Edwardsiella tarda is a bacterial pathogen that can infect both humans and animals. TX1, an Ed. tarda strain isolated from diseased fish, was found to produce autoinducer 2 (Al-2)-like activity that was growth phase dependent and modulated by growth conditions. The gene coding for the Al-2 synthase was cloned from TX1 and designated luxS(Et). LuxS(Et) was able to complement the Al-2 mutant phenotype of Escherichia coli strain DH5 alpha. Expression Of luxS(Et) correlated with Al-2 activity and was increased by glucose and decreased by elevated temperature. The effect of glucose was shown to be mediated through the cAMP-CRP complex, which repressed luxS(Et) expression. Overexpression of luxS(Et) enhanced Al-2 activity in TX1, whereas disruption of luxS(Et) expression by antisense RNA interference (i) reduced the level of Al-2 activity, (ii) impaired bacterial growth under various conditions, (iii) weakened the expression of genes associated with the type III secretion system and biofilm formation, and (iv) attenuated bacterial virulence. Addition of exogenous Al-2 was able to complement the deficiencies in the expression of TTSS genes and biofilm production but failed to rescue the growth defects. Our results (i) demonstrated that the Al-2 activity in TX1 is controlled at least in part at the level of luxS(Et) expression, which in turn is regulated by growth conditions, and that the temporal expression of luxS(Et) is essential for optimal bacterial infection and survival; and (ii) suggested the existence in Ed. tarda of a LuxS/Al-2-mediated signal transduction pathway that regulates the production of virulence-associated elements. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1722]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Sun, Kun,Sun, Li,et al. Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain[J]. MICROBIOLOGY-SGM,2008,154(Part 7):2060-2069. |
| APA | Zhang, Min,Sun, Kun,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2008).Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain.MICROBIOLOGY-SGM,154(Part 7),2060-2069. |
| MLA | Zhang, Min,et al."Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain".MICROBIOLOGY-SGM 154.Part 7(2008):2060-2069. |
入库方式： OAI收割
来源：海洋研究所
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