Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong
文献类型：期刊论文
| 作者 | Zhang, Rui ; Liu, Baozhong; Lau, Stanley C. K.; Ki, Jang-Seu; Qian, Pei-Yuan; Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China
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| 刊名 | FEMS MICROBIOLOGY ECOLOGY
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| 出版日期 | 2007-09-01 |
| 卷号 | 61期号:3页码:496-508 |
| 关键词 | Particle-attached Free-living Contrasting Marine Environment |
| ISSN号 | 0168-6496 |
| DOI | 10.1111/j.1574-6941.2007.00353.x |
| 文献子类 | Article |
| 英文摘要 | Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.; Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000248961900010 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1726]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Rui,Liu, Baozhong,Lau, Stanley C. K.,et al. Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong[J]. FEMS MICROBIOLOGY ECOLOGY,2007,61(3):496-508. |
| APA | Zhang, Rui,Liu, Baozhong,Lau, Stanley C. K.,Ki, Jang-Seu,Qian, Pei-Yuan,&Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China.(2007).Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong.FEMS MICROBIOLOGY ECOLOGY,61(3),496-508. |
| MLA | Zhang, Rui,et al."Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong".FEMS MICROBIOLOGY ECOLOGY 61.3(2007):496-508. |
入库方式： OAI收割
来源：海洋研究所
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